Experimental Orthopaedics, Medical University Innsbruck, Innsbruck, Austria.
J Orthop Res. 2012 Jul;30(7):1176-80. doi: 10.1002/jor.22065. Epub 2012 Jan 6.
The bacteria in implant-related infections can evade host defenses by forming biofilms. The more we understand biofilm behavior, the better we can fight against then clinically. In vitro models for biofilms allow tests simulating in vivo conditions. In this study we evaluated the Minimum Biofilm Eradication Concentration-High Throughput Plates (MBEC™-HTP) as biofilm in vitro model for studies of implant associated infections. Staphylococcus aureus and Staphylococcus epidermidis biofilms were grown on MBEC™-HTP. To ensure the biofilm formation, antibiotic susceptibility tests and scanning electron microscopy (SEM) was carried out. Susceptibility tests were carried out using gentamicin, vancomycin, rifampicin, fosfomycin, clindamycin, and linezolid. Colony forming units counting were carried out. Minimal inhibitory concentration (MIC) and biofilm inhibitory concentration (BIC) were estimated. The CFU counting showed potency of rifampicin and daptomycin against S. epidermidis biofilms and rifampicin against S. aureus biofilms. SEM images showed proteic material in contact with cells. The differences between BIC and MIC demonstrated the biofilm formation as well as the SEM images. Rifampicin and daptomycin are good choices against biofilm related infections. Moreover, after suggested modifications, the model used in this study is eligible to further studies of implant associated infections.
植入物相关感染中的细菌可以通过形成生物膜来逃避宿主防御。我们对生物膜行为的了解越多，就能在临床上更好地对抗它们。体外生物膜模型可以模拟体内条件进行测试。在这项研究中，我们评估了最低抑菌浓度高通量平板（MBEC™-HTP）作为研究植入物相关感染的体外生物膜模型。金黄色葡萄球菌和表皮葡萄球菌生物膜在 MBEC™-HTP 上生长。为了确保生物膜的形成，进行了抗生素敏感性测试和扫描电子显微镜（SEM）检查。使用庆大霉素、万古霉素、利福平、磷霉素、克林霉素和利奈唑胺进行了药敏试验。进行了集落形成单位计数。估计了最小抑菌浓度（MIC）和生物膜抑制浓度（BIC）。CFU 计数显示利福平对表皮葡萄球菌生物膜和利福平对金黄色葡萄球菌生物膜具有杀菌作用。SEM 图像显示了与细胞接触的蛋白质物质。BIC 和 MIC 之间的差异表明了生物膜的形成以及 SEM 图像。利福平利福平和达托霉素是治疗生物膜相关感染的良好选择。此外，经过建议的修改后，本研究中使用的模型有资格进行进一步的植入物相关感染研究。
