Spiegel Christopher, Coraça-Huber Débora C, Nogler Michael, Arora Rohit, Putzer David
Research Laboratory for Biofilms and Implant Associated Infections (BIOFILM LAB), Experimental Orthopaedics, University Hospital for Orthopaedics and Traumatology, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.
Department of Orthopaedics and Traumatology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, Austria.
Pathogens. 2024 Dec 28;14(1):10. doi: 10.3390/pathogens14010010.
Periprosthetic joint infections occur in 1-2% of all patients undergoing prosthetic joint surgeries. Although strong efforts have been made to reduce infection rates, conventional therapies like one- or two-stage revisions have failed to lower the infection rates. Cold atmospheric plasma (CAP) has shown promising results in reducing bacterial loads on surfaces. In this study, we aimed to investigate the ability of CAP to reduce the bacterial load on metal surfaces with varying distances and different plasma compositions below a temperature suitable for in vivo applications. Methods: Biofilm was formed with ATCC 29213 and ATCC 12228 cultures on TMZF discs. Plasma treatments using air plasma and argon plasma were conducted on discs containing the established biofilm while the temperature was measured. During the experiments, the duration and the distance of plasma application varied. Afterwards, colony-forming units were counted. Results: The results of this study showed that air and argon plasma could be considered for applications during surgeries at a 1 cm distance. While air plasma showed the highest efficiency in CFU reduction, the temperature generation due to the presence of oxygen poses a limitation concerning the duration of application. The use of argon as a plasma generator does not show the temperature limitation in correlation to exposure time. The use of air plasma with a distance of 1 cm to the application site and an exposure time of 5 s showed the most effective bacterial reduction while not exceeding tissue-damaging temperatures.
人工关节周围感染发生在所有接受人工关节手术患者中的1%-2%。尽管已经做出了巨大努力来降低感染率，但像一期或二期翻修这样的传统治疗方法未能降低感染率。低温大气等离子体（CAP）在减少表面细菌负荷方面已显示出有前景的结果。在本研究中，我们旨在研究CAP在低于适合体内应用的温度下，在不同距离和不同等离子体组成的情况下，降低金属表面细菌负荷的能力。方法：在TMZF圆盘上用ATCC 29213和ATCC 12228培养物形成生物膜。在含有已形成生物膜的圆盘上进行空气等离子体和氩等离子体处理，并测量温度。在实验过程中，等离子体应用的持续时间和距离有所变化。之后，对菌落形成单位进行计数。结果：本研究结果表明，空气和氩等离子体可考虑在手术中距离为1 cm时应用。虽然空气等离子体在减少CFU方面显示出最高效率，但由于氧气存在而产生的温度对应用持续时间构成了限制。使用氩作为等离子体发生器在与暴露时间相关的方面未显示出温度限制。在距离应用部位1 cm且暴露时间为5 s的情况下使用空气等离子体显示出最有效的细菌减少效果，同时不超过组织损伤温度。
