激活水稻尼克酸合成酶 2（OsNAS2）可提高生物强化的铁供应。
Activation of Rice nicotianamine synthase 2 (OsNAS2) enhances iron availability for biofortification.
机构信息
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA.
出版信息
Mol Cells. 2012 Mar;33(3):269-75. doi: 10.1007/s10059-012-2231-3. Epub 2012 Jan 3.
Abstract
Because micronutrients in human diets ultimately come from plant sources, malnutrition of essential minerals is a significant public health concern. By increasing the expression of nicotianamine synthase (NAS), we fortified the level of bioavailable iron in rice seeds. Activation of iron deficiency-inducible OsNAS2 resulted in a rise in Fe content (3.0-fold) in mature seeds. Its ectopic expression also increased that content. Enhanced expression led to higher tolerance of Fe deficiency and better growth under elevated pH. Mice fed with OsNAS2-D1 seeds recovered more rapidly from anemia, indicating that bioavailable Fe contents were improved by this increase in OsNAS2 expression.
摘要
由于人类饮食中的微量营养素最终来自植物来源，因此必需矿物质的营养不良是一个重大的公共卫生问题。通过增加植物螯合肽合成酶（NAS）的表达，我们增强了水稻种子中生物可利用铁的水平。铁饥饿诱导 OsNAS2 的激活导致成熟种子中铁含量（增加 3 倍）升高。其异位表达也增加了铁的含量。增强表达导致铁缺乏的耐受性更高，在 pH 值升高的情况下生长更好。用 OsNAS2-D1 种子喂养的老鼠从贫血中恢复得更快，这表明通过增加 OsNAS2 的表达，生物可利用铁的含量得到了改善。
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2
Nicotianamine, a novel enhancer of rice iron bioavailability to humans.
PLoS One. 2010 Apr 16;5(4):e10190. doi: 10.1371/journal.pone.0010190.
3
Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese.
Plant J. 2010 May;62(3):379-90. doi: 10.1111/j.1365-313X.2010.04158.x. Epub 2010 Feb 1.
4
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5
Rice endosperm iron biofortification by targeted and synergistic action of nicotianamine synthase and ferritin.
Plant Biotechnol J. 2009 Sep;7(7):631-44. doi: 10.1111/j.1467-7652.2009.00430.x.
6
Critical evaluation of strategies for mineral fortification of staple food crops.
Transgenic Res. 2010 Apr;19(2):165-80. doi: 10.1007/s11248-009-9311-y. Epub 2009 Aug 15.
7
Disruption of OsYSL15 leads to iron inefficiency in rice plants.
Plant Physiol. 2009 Jun;150(2):786-800. doi: 10.1104/pp.109.135418. Epub 2009 Apr 17.
8
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Plant Cell Environ. 2009 Apr;32(4):408-16. doi: 10.1111/j.1365-3040.2009.01935.x. Epub 2009 Jan 14.
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