JTN Wong Laboratory for Bone Tissue Engineering, 740 Ave Dr Penfield, Montreal, Quebec, Canada.
J Orthop Res. 2012 Aug;30(8):1183-9. doi: 10.1002/jor.22028. Epub 2012 Jan 6.
An overall decline in the availability of osteogenic precursor cells and growth factors in the bone marrow microenvironment have been associated with impaired bone formation and osteopenia in humans. The objective of the current study was to determine if transplantation of mesenchymal stromal cells (MSC) from a healthy, young donor mouse into an osteopenic recipient mouse could enhance osseointegration of a femoral implant. MSC harvested from normal young adult mice differentiated into bone forming osteoblasts when cultured on implant grade titanium surfaces ex vivo and promoted bone formation around titanium-coated rods implanted in the femoral canal of osteopenic recipient mice. Micro computed tomographic imaging and histological analyses showed more, better quality, bone in the femur that received the MSC transplant compared with the contra-lateral control femur that received carrier alone. These results provide pre-clinical evidence that MSC transplantation promotes peri-implant bone regeneration and suggest the approach could be used in a clinical setting to enhance bone regeneration and healing in patients with poor quality bone.
骨髓微环境中成骨前体细胞和生长因子的整体减少与人类骨形成受损和骨质疏松症有关。本研究的目的是确定将来自健康年轻供体小鼠的间充质基质细胞 (MSC) 移植到骨质疏松症受体小鼠体内是否可以增强股骨植入物的骨整合。从正常年轻成年小鼠中分离出的 MSC 在植入级钛表面上进行体外培养时可分化为成骨细胞，促进骨质疏松症受体小鼠股骨管内钛涂层棒周围的骨形成。微计算机断层扫描成像和组织学分析显示，接受 MSC 移植的股骨中骨量更多，质量更好，与接受载体的对侧对照股骨相比。这些结果提供了临床前证据，证明 MSC 移植可促进种植体周围骨再生，并表明该方法可用于临床环境，以增强骨量差的患者的骨再生和愈合。
