The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Woods Hole, MA, USA.
Biotechniques. 2012 Jan;52(1):39-43. doi: 10.2144/000113802.
The selection, isolation, and accurate positioning of single cells in three dimensions are increasingly desirable in many areas of cell biology and tissue engineering. We describe the application of a simple and low cost dielectrophoretic device for picking out and relocating single target cells. The device consists of a single metal electrode and an AC signal generator. It does not require microfabrication technologies or sophisticated electronics. The dielectrophoretic manipulator also discriminates between live and dead cells and is capable of redistributing intracellular organelles.
在细胞生物学和组织工程的许多领域，越来越需要对单细胞进行选择、分离和精确定位。我们描述了一种简单且低成本的介电泳装置在挑选和重新定位单个靶细胞方面的应用。该装置由单个金属电极和交流信号发生器组成，不需要微制造技术或复杂的电子设备。介电泳操作器还可以区分活细胞和死细胞，并能够重新分配细胞内细胞器。
