Institute for Integrated Micro and Nano Systems, Joint Research Institute for Integrated Systems, School of Engineering, Edinburgh, University of Edinburgh, Edinburgh EH9 3JF, UK.
IET Nanobiotechnol. 2011 Mar;5(1):1-7. doi: 10.1049/iet-nbt.2010.0010.
The ability to isolate and accurately position single cells in three dimensions is becoming increasingly important in many areas of biological research. The authors describe the design, theoretical modelling and testing of a novel dielectrophoretic (DEP) tweezer for picking out and relocating single target cells. The device is constructed using facilities available in most electrophysiology laboratories, without the requirement of sophisticated and expensive microfabrication technology, and offers improved practical features over previously reported DEP tweezer designs. The DEP tweezer has been tested using transfected HEI-193 human schwannoma cells, with visual identification of the target cells being aided by labelling the incorporated gene product with a green fluorescent protein.
在许多生物研究领域，能够在三维空间中分离和准确定位单细胞变得越来越重要。本文作者描述了一种新型的介电泳（DEP）镊子的设计、理论建模和测试，用于挑取和重新定位单个靶细胞。该装置使用大多数电生理学实验室都具备的设备构建，无需复杂且昂贵的微制造技术，并且相对于以前报道的DEP 镊子设计具有改进的实际功能。该 DEP 镊子已使用转染的 HEI-193 人施万细胞瘤进行了测试，通过用绿色荧光蛋白标记所掺入的基因产物，辅助对靶细胞进行目视识别。
