Pethig R, Markx G H
Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.
Trends Biotechnol. 1997 Oct;15(10):426-32. doi: 10.1016/S0167-7799(97)01096-2.
Recent progress in the development of microelectrode structures has led to new techniques for the dielectrophoretic characterization and sorting of cells, microorganisms and other bioparticles using nonuniform AC electric fields. These methods utilize differences in the dielectric polarizabilities of cells for their effectiveness, and factors controlling such properties include the conductivity and permittivity of membranes and any cell walls, electrical double layers associated with surface charges, cell morphologies, and internal structures. Applications of dielectrophoresis have included the selective spatial manipulation and separation of mixtures of bacteria, viable and unviable cells, cancerous and normal cells, and red and white blood cells.
微电极结构开发方面的最新进展带来了新技术，可利用非均匀交流电场对细胞、微生物及其他生物颗粒进行介电电泳表征和分选。这些方法利用细胞介电极化率的差异来发挥作用，控制此类特性的因素包括细胞膜及任何细胞壁的电导率和介电常数、与表面电荷相关的双电层、细胞形态和内部结构。介电电泳的应用包括对细菌混合物、活细胞与死细胞、癌细胞与正常细胞以及红细胞与白细胞进行选择性空间操控和分离。
