Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States.
Langmuir. 2012 Jan 24;28(3):1689-93. doi: 10.1021/la2047257. Epub 2012 Jan 11.
A new amino acid derived fluorescent linker for attaching molecules to the surface of a microelectrode array has been developed. Molecules to be monitored on an array are attached to the C-terminus of the linker, the N-terminus is then used to attach the linker to the array, and the side chain is used to synthesize a fluorescent tag. The fluorescent group is made with a one-step oxidative cycloaddition reaction starting from a hydroxyindole group. The linker is compatible with site-selective Cu(I)-chemistry on the array, it allows for quality control assessment of the array itself, and it is compatible with the electrochemical impedance experiments used to monitor binding events on the surface of the array.
已经开发出一种新的衍生氨基酸荧光接头，用于将分子连接到微电极阵列的表面。要在阵列上监测的分子连接到接头的 C 末端，然后将 N 末端用于将接头连接到阵列，侧链用于合成荧光标记物。荧光团通过从羟基吲哚基团开始的一步氧化环加成反应制成。接头与阵列上的选择性 Cu（I）化学相容，它允许对阵列本身进行质量控制评估，并且与用于监测阵列表面结合事件的电化学阻抗实验兼容。
