Khandjian E W
Centre de recherche, Hôpital St-François d'Assise, Québec, Canada.
Biochem Cell Biol. 1990 Apr;68(4):804-7. doi: 10.1139/o90-116.
Exposure of primary mouse kidney cell cultures to acidic medium (pH 5.5) induced the expression of a 70 kilodalton (kDa) protein. This protein was identified as the major inducible heat-shock protein 70 (hsp70) by immunoprecipitation with anti-hsp70 serum and Northern blot analysis with a hsp70 cDNA probe. Maximum induction of the 70-kDa protein at pH 5.5 after 240 min was about 30% of that observed after 60 min of thermal treatment at 43 degrees C. In addition, there was an apparent induction of the glucose-regulated proteins (GRPs) of 76-78 and 98-100 kDa, but not of the other hsps. This subset induction of the heat-shock response by acidic medium suggests that different mechanisms are responsible for the induction of the various families of hsps.
将原代小鼠肾细胞培养物暴露于酸性培养基（pH 5.5）中会诱导一种70千道尔顿（kDa）蛋白质的表达。通过用抗hsp70血清进行免疫沉淀以及用hsp70 cDNA探针进行Northern印迹分析，该蛋白质被鉴定为主要的诱导型热休克蛋白70（hsp70）。在240分钟后，pH 5.5条件下70-kDa蛋白质的最大诱导量约为43℃热处理60分钟后观察到的诱导量的30%。此外，明显诱导了76 - 78 kDa和98 - 100 kDa的葡萄糖调节蛋白（GRP），但其他热休克蛋白未被诱导。酸性培养基对热休克反应的这种亚组诱导表明，不同的机制负责诱导不同家族的热休克蛋白。
