Nishimura R N, Dwyer B E, Cole R, de Vellis J, Picard K
Department of Neurology, Veterans Administration Hospital, Sepulveda, California 91343.
Exp Cell Res. 1989 Jan;180(1):276-80. doi: 10.1016/0014-4827(89)90231-0.
Cultured rat astrocytes were exposed for 1 or 3 h to acidic medium (pH adjusted to 5.0, 5.5, or 6.0). Radioactive labeling for 3 h after exposure to acidic medium revealed increased synthesis of many proteins, including an inducible 68-kDa protein. Optimal extracellular (medium) pH for the induction of this 68-kDa protein was 5.5. Immunoblotting demonstrated that this 68-kDa protein induced by acidosis was the 68-kDa heat-shock protein previously described in cultured astrocytes.
将培养的大鼠星形胶质细胞置于酸性培养基（pH值调至5.0、5.5或6.0）中1或3小时。在暴露于酸性培养基后进行3小时的放射性标记，结果显示许多蛋白质的合成增加，包括一种诱导性68 kDa蛋白。诱导这种68 kDa蛋白的最佳细胞外（培养基）pH值为5.5。免疫印迹表明，酸中毒诱导的这种68 kDa蛋白是先前在培养的星形胶质细胞中描述的68 kDa热休克蛋白。
