Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
BMC Genomics. 2012 Jan 10;13:10. doi: 10.1186/1471-2164-13-10.
The transcriptome of the fruit pulp of the sweet orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening.
The transcriptomes of WT and MT were sampled at four developmental stages using an Illumina sequencing platform. A total of 19,440 and 18,829 genes were detected in MT and WT, respectively. Hierarchical clustering analysis revealed 24 expression patterns for the set of all genes detected, of which 20 were in common between MT and WT. Over 89% of the genes showed differential expression during fruit development and ripening in the WT. Functional categorization of the differentially expressed genes revealed that cell wall biosynthesis, carbohydrate and citric acid metabolism, carotenoid metabolism, and the response to stress were the most differentially regulated processes occurring during fruit development and ripening.
A description of the transcriptomic changes occurring during fruit development and ripening was obtained in sweet orange, along with a dynamic view of the gene expression differences between the wild type and a red fleshed mutant.
比较甜橙品种安流（WT）和其红肉突变体红安流（MT）的果肉转录组，以了解在果实发育和成熟过程中基因表达的动态和差异表达。
使用 Illumina 测序平台在四个发育阶段分别对 WT 和 MT 的转录组进行采样。在 MT 和 WT 中分别检测到 19440 个和 18829 个基因。层次聚类分析显示，检测到的所有基因的集合有 24 种表达模式，其中 20 种在 MT 和 WT 之间是共同的。WT 中超过 89%的基因在果实发育和成熟过程中表现出差异表达。差异表达基因的功能分类表明，细胞壁生物合成、碳水化合物和柠檬酸代谢、类胡萝卜素代谢以及对胁迫的反应是果实发育和成熟过程中差异调控最明显的过程。
获得了甜橙果实发育和成熟过程中转录组的变化描述，以及野生型和红肉突变体之间基因表达差异的动态视图。
