Department d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007 Tarragona, Spain.
Biosens Bioelectron. 2012 Mar 15;33(1):134-8. doi: 10.1016/j.bios.2011.12.040. Epub 2011 Dec 27.
An electrochemical immunosensor for the detection of human IgA deficiency in real human blood serum has been developed. The performance of the immunosensor presents a large but sensitive dynamic range that allows the determination of non-deficient IgA levels (>70 μg/mL) as well as of severe IgA deficiencies (0.5-5.0 μg/mL). The assay architecture involves the immobilisation of a coating antibody on an electrode surface using carboxylic-ended bipodal alkane-thiol self-assembled monolayers (SAMs). The long chain bipodal SAM presents intercalated poly(ethylenglycol) groups that confer the immunosensor the ability to retain its optimum performance in very complex matrices and serum with negligible non-specific adsorption phenomena. Amperometric optimisation of the assay resulted in limits of detection of 142 ng/mL in just 30 min total assay time. Real patients' serum samples were analysed using the developed electrochemical immunosensor demonstrating an excellent correlation in terms of sensitivity and reproducibility compared with standard enzyme linked immunosorbent assays (ELISA).
已经开发出一种用于检测人血清中 IgA 缺乏症的电化学免疫传感器。该免疫传感器的性能呈现出较大但敏感的动态范围，允许测定非缺乏 IgA 水平（>70μg/mL）以及严重 IgA 缺乏症（0.5-5.0μg/mL）。该测定架构涉及使用羧酸端双足烷硫醇自组装单层（SAM）将涂层抗体固定在电极表面上。长链双足 SAM 呈现出嵌入的聚（乙二醇）基团，赋予免疫传感器在非常复杂的基质和血清中保持最佳性能的能力，而几乎没有非特异性吸附现象。通过安培法对测定进行优化，在总测定时间仅 30 分钟的情况下，检测限达到 142ng/mL。使用开发的电化学免疫传感器分析了真实患者的血清样本，与标准酶联免疫吸附测定（ELISA）相比，在灵敏度和重现性方面具有出色的相关性。
