Laboratoire de Virologie, Centre National de Référence des Arbovirus et Virus Influenza Région Antilles-Guyane, Institut Pasteur de la Guyane, Cayenne, French Guiana.
Am J Trop Med Hyg. 2012 Jan;86(1):159-65. doi: 10.4269/ajtmh.2012.11-0475.
To strengthen active dengue surveillance in Saint Martin and Saint Barthélemy, two French Caribbean islands, we evaluated the epidemiological usefulness of collecting blood samples from NS1-positive dengue patients on filter paper to identify the dengue serotypes circulating in these regions during a 27-month period. This approach allowed dengue serotypes to be identified by reverse transcriptase-polymerase chain reaction in 90.1% of the total set of 666 samples analyzed and, in 95.5% of the samples collected during the acute phase of the disease. This prospective virological surveillance using blood samples absorbed onto filter paper, which were stored at 4°C and shipped at ambient temperature to a specialized laboratory for analysis, allowed us to avoid the logistic and financial costs associated with shipping frozen venous blood samples. This surveillance system offers a low-cost alternative for reinforcing dengue prevention in areas where specialized laboratories do not exist, notably by facilitating the early detection of potentially new dengue serotypes.
为加强圣马丁和圣巴泰勒米（法属加勒比地区的两个岛屿）的登革热主动监测，我们评估了采集 NS1 阳性登革热患者滤纸血样的方法在识别这些地区流行登革热血清型方面的流行病学价值，为期 27 个月。这种方法通过逆转录-聚合酶链反应在 666 份分析样本的 90.1%和疾病急性期采集的 95.5%的样本中鉴定出了登革热血清型。这种使用滤纸上吸附的血样进行前瞻性病毒学监测，将样本储存在 4°C 并在室温下运送到专门实验室进行分析，避免了与运输冷冻静脉血样本相关的后勤和财务成本。该监测系统为不存在专门实验室的地区加强登革热预防提供了一种低成本的替代方法，特别是有助于早期发现可能的新登革热血清型。
