Matheus Severine, Meynard Jean-Baptiste, Lavergne Anne, Girod Romain, Moua David, Labeau Bhety, Dussart Philippe, Lacoste Vincent, Deparis Xavier
Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana.
Am J Trop Med Hyg. 2008 Nov;79(5):685-7.
During a dengue-3 outbreak in Paraguay at the beginning of 2007, capillary blood samples absorbed onto filter papers were collected from 44 suspected cases. These samples were subjected to three molecular and serologic tests, and 31 of the 44 samples gave a positive result by at least one of the techniques used. Molecular analyses detected the dengue-3 serotype in 22 patients and additionally the dengue-2 serotype in two patients. Therefore two different serotypes were co-circulating during this outbreak. Overall, this study validates the use of dried-blood samples for field screening investigations. Indeed, all types of laboratory studies of dengue were possible with samples consisting of a few drops of dried blood from finger pricks.
2007年初巴拉圭发生登革热3型疫情期间，从44例疑似病例中采集了吸附在滤纸上的毛细血管血样。这些样本接受了三项分子和血清学检测，44份样本中有31份通过至少一种所使用的技术检测呈阳性。分子分析在22名患者中检测到登革热3型血清型，另外在两名患者中检测到登革热2型血清型。因此，在此次疫情期间两种不同的血清型共同传播。总体而言，本研究验证了干血样本用于现场筛查调查的可行性。事实上，用手指刺破采集的几滴干血样本可以进行各类登革热实验室研究。
