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[Purification and physi-chemical properties of polysaccharides SJZPS-Vb-1-2 from immunocompetence parts of sijunzi decoction].

作者信息

Zhu Chenchen, Gui Shuhua, Wang Peixun, Chen Weiwen

机构信息

Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.

出版信息

Zhong Yao Cai. 2003 Oct;26(10):751-3.

PMID:14768397
Abstract

OBJECTIVE

To supply basic research materials for clarifying the immunocompetence materials of Sijunzi Decoction.

METHODS

Two polysaccharides(SJZPS-Vb-1-2) were purified with Sephadex DEAE A 25 and Sephadex G 200 from SJZPS-Vb part (with the highest immunocompetence). Their molecular weights were determined. The sugar compositions were analyzed with GC. The linkage positions of the component sugars were determined by methylation and GC/MS.

RESULTS

SJZPS-Vb-1-2 was uonic-containing heteropolysaccharides with the component sugars of glucose, galactose and mannose in different molar ratios. The molecular weight of SJZPS-VB-1 was 38,300, SJZPS-VB-2, 26,000. The molar ratio of SJZPS-Vb-1 was as glu:gal:man 1:046:2.15 and SJZPS-Vb-2, glu:gal:man 1:1.41:4.18.

摘要

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