Department of Ophthalmology, The First Hospital of China Medical University, Shenyang, China.
J Ocul Pharmacol Ther. 2012 Aug;28(4):397-401. doi: 10.1089/jop.2011.0168. Epub 2012 Jan 10.
To determine the aqueous humor levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in the eyes of patients with Coats' disease and study the correlation between these levels.
Samples of aqueous humor were obtained from 7 patients with Coats' disease and 15 age-matched patients with congenital cataracts as controls. Nitrite and nitrate (NOx), the stable end products of NO, were detected by the Griess reaction, and VEGF levels were assessed by enzyme-linked immunosorbent assay.
The aqueous humor NOx and VEGF levels were elevated in the eyes of patients with Coats' disease compared with those of controls (P=0.001 and P<0.001, respectively). The median NOx level was 55.2 μM (range, 23.0-75.3 μM) in the Coats' disease group and 18.8 μM (range, 8.7-36.2 μM) in the control group. The median VEGF level was 731.7 pg/mL (range, 288.3-1364.3 pg/mL) in the Coats' disease group and 33.3 pg/mL (range, 9.0-96.8 pg/mL) in the control group. No correlation was observed between the aqueous humor concentrations of NOx and VEGF.
NOx and VEGF are increased but not related in the aqueous humor samples of patients with Coats' disease. NO and VEGF may play roles in the pathogenesis of Coats' disease. Further studies are needed to clearly elucidate the relationship among VEGF, NO, and other cytokines in Coats' disease.
测定 coats 病患者眼内房水中一氧化氮（NO）和血管内皮生长因子（VEGF）的水平，并研究两者之间的相关性。
收集 7 例 coats 病患者和 15 例年龄匹配的先天性白内障患者的房水样本作为对照。通过格里塞反应检测房水中的硝酸盐和亚硝酸盐（NOx），这是 NO 的稳定终产物，并用酶联免疫吸附试验测定 VEGF 水平。
与对照组相比，coats 病患者眼内房水中的 NOx 和 VEGF 水平升高（P=0.001 和 P<0.001）。coats 病组的 NOx 中位数为 55.2 μM（范围，23.0-75.3 μM），对照组为 18.8 μM（范围，8.7-36.2 μM）。coats 病组的 VEGF 中位数为 731.7 pg/mL（范围，288.3-1364.3 pg/mL），对照组为 33.3 pg/mL（范围，9.0-96.8 pg/mL）。但在 coats 病患者的房水中，NOx 和 VEGF 的浓度之间没有相关性。
NOx 和 VEGF 在 coats 病患者的房水中升高，但没有相关性。NO 和 VEGF 可能在 coats 病的发病机制中起作用。需要进一步的研究来阐明 coats 病中 VEGF、NO 和其他细胞因子之间的关系。
