School of Pharmacy, Shanghai Jiao Tong University, Shanghai, PR China.
Planta Med. 2012 Mar;78(5):465-71. doi: 10.1055/s-0031-1298147. Epub 2012 Jan 10.
Four new sesquiterpene lactones, (1 S,5 R,6 S,7 S,8 R,9 R,10 S,11 S)-6-acetoxy-9-hydroxy-4-oxo-pseudoguai-2(3)-en-12,8-olide, (1 S,2 R,5 R,6 S,7 R,8 S,10 R)-6-acetoxy-2-ethoxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, (1 S,2 R,5 R,6 S,7 R,8 S,10 R)-6-acetoxy-2-hydroxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, and 14-acetoxy-1 β,5 α,7 αH-4 β-hydroxy-guai-9(10),11(13)-dien-12,8 α-olide, along with 26 known sesquiterpene lactones, were isolated from the whole plants of Inula hookeri C. B. Clarke. Their structures were established based on spectroscopic methods including HRESIMS, 1D and 2D NMR, and CD techniques. All compounds were evaluated for their cytotoxic activities against HepG2, HeLa, PC-3, and MGC-803 cell lines by CCK-8 assay. Some of the isolates, especiallly pseudoguaianolides and guaianolides, exhibited significant cytotoxicities against these four examined cell lines.
从旋覆花（Inula hookeri C. B. Clarke）全草中分离得到了四个新的倍半萜内酯化合物：（1 S,5 R,6 S,7 S,8 R,9 R,10 S,11 S）-6-乙酰氧基-9-羟基-4-氧代伪土木香内酯-2(3)-烯-12,8-内酯、（1 S,2 R,5 R,6 S,7 R,8 S,10 R）-6-乙酰氧基-2-乙氧基-4-氧代伪土木香-11(13)-烯-12,8-内酯、（1 S,2 R,5 R,6 S,7 R,8 S,10 R）-6-乙酰氧基-2-羟基-4-氧代伪土木香-11(13)-烯-12,8-内酯和 14-乙酰氧基-1 β,5 α,7 αH-4 β-羟基-愈创木-9(10),11(13)-二烯-12,8 α-内酯，以及 26 个已知的倍半萜内酯化合物。通过高分辨质谱（HRESIMS）、1D 和 2D NMR 以及 CD 技术等光谱方法确定了它们的结构。采用 CCK-8 法评价了所有化合物对 HepG2、HeLa、PC-3 和 MGC-803 细胞系的细胞毒性。一些分离物，特别是伪土木香内酯和土木香内酯，对这四种细胞系表现出显著的细胞毒性。
