Department of Oncology, Shanghai East Hospital, Tongji University, Shanghai, 200120, China.
Clin Exp Med. 2013 Feb;13(1):11-9. doi: 10.1007/s10238-011-0174-1. Epub 2012 Jan 11.
To study the inhibitory effect of nuclear transcription factor-kappa B(NF-κB) antisense oligodeoxynucleotide(ASODN) on the growth and tumorgenesis of human gastric cancer. We synthesized and transfected the ASODN of NF-κB/P65 to gastric cancer cell line. The effect of ASODN of NF-κB/P65 on the proliferation of gastric cancer cells was measured by MTT method. The subcutaneous xenograft model of human gastric cancer was established in nude mice, and the tumor growth curve was observed. The cell proliferation was significantly inhibited in P65 ASODN-transfected group in vitro (P<0.05). In vivo, tumor formation test showed that the tumor volume in nude mice in ASODN group was obviously smaller than in other groups (P<0.05); the apoptosis index (AI) was significantly higher (P<0.001). Simultaneously, MVD in ASODN group was markedly lower than in other groups (P<0.01). NF-κB could be used as a new biological therapeutic target of gastric cancer.
为了研究核转录因子-κB（NF-κB）反义寡脱氧核苷酸（ASODN）对人胃癌生长和致瘤的抑制作用。我们合成并转染了 NF-κB/P65 的 ASODN 至胃癌细胞系。通过 MTT 法测量 ASODN 对胃癌细胞增殖的影响。在裸鼠中建立人胃癌皮下移植瘤模型，观察肿瘤生长曲线。体外 P65 ASODN 转染组细胞增殖明显受到抑制（P<0.05）。在体内，肿瘤形成试验表明，ASODN 组裸鼠肿瘤体积明显小于其他组（P<0.05）；凋亡指数（AI）显著升高（P<0.001）。同时，ASODN 组的 MVD 明显低于其他组（P<0.01）。NF-κB 可作为胃癌的一个新的生物治疗靶点。
