Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
J Hered. 2012 May-Jun;103(3):459-64. doi: 10.1093/jhered/esr139. Epub 2012 Jan 10.
Genetic analyses using ancient DNA from Pleistocene and early Holocene fossils have largely relied on mitochondrial DNA (mtDNA) sequences. Among woolly mammoths, Mammuthus primigenius, mtDNA analyses have identified 2 distinct clades (I and II) that diverged 1-2 Ma. Here, we establish that microsatellite markers can be effective on Pleistocene samples, successfully genotyping woolly mammoth specimens at 2 loci. Although significant differentiation at the 2 microsatellite loci was not detected between 16 clade I and 4 clade II woolly mammoths, our results demonstrate that the nuclear population structure of Pleistocene species can be examined using fast-evolving nuclear microsatellite markers.
利用更新世和早全新世化石中的古 DNA 进行的遗传分析在很大程度上依赖于线粒体 DNA（mtDNA）序列。在猛犸象中，猛犸象属已鉴定出 2 个不同的分支（I 和 II），它们在 1-2 百万年前分化。在这里，我们证明微卫星标记可以有效地用于更新世样本，成功地在 2 个基因座对猛犸象标本进行了基因分型。尽管在 16 个分支 I 和 4 个分支 II 的猛犸象之间没有检测到 2 个微卫星基因座的显著分化，但我们的结果表明，可以使用快速进化的核微卫星标记来检测更新世物种的核种群结构。
