ANSES, Dozulé Laboratory for Equine Diseases, Dozulé, France.
PLoS One. 2012;7(1):e29953. doi: 10.1371/journal.pone.0029953. Epub 2012 Jan 3.
The Taylorella genus comprises two species: Taylorella equigenitalis, which causes contagious equine metritis, and Taylorella asinigenitalis, a closely-related species mainly found in donkeys. We herein report on the first genome sequence of T. asinigenitalis, analyzing and comparing it with the recently-sequenced T. equigenitalis genome. The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3% GC content and 1,534 coding sequences (CDS). While 212 CDSs were T. asinigenitalis-specific, 1,322 had orthologs in T. equigenitalis. Two hundred and thirty-four T. equigenitalis CDSs had no orthologs in T. asinigenitalis. Analysis of the basic nutrition metabolism of both Taylorella species showed that malate, glutamate and alpha-ketoglutarate may be their main carbon and energy sources. For both species, we identified four different secretion systems and several proteins potentially involved in binding and colonization of host cells, suggesting a strong potential for interaction with their host. T. equigenitalis seems better-equipped than T. asinigenitalis in terms of virulence since we identified numerous proteins potentially involved in pathogenicity, including hemagluttinin-related proteins, a type IV secretion system, TonB-dependent lactoferrin and transferrin receptors, and YadA and Hep_Hag domains containing proteins. This is the first molecular characterization of Taylorella genus members, and the first molecular identification of factors potentially involved in T. asinigenitalis and T. equigenitalis pathogenicity and host colonization. This study facilitates a genetic understanding of growth phenotypes, animal host preference and pathogenic capacity, paving the way for future functional investigations into this largely unknown genus.
引起马传染性子宫炎的马泰勒菌和主要存在于驴中的近缘种羊泰勒菌。我们在此报告了羊泰勒菌的首个基因组序列，对其进行了分析，并与最近测序的马泰勒菌基因组进行了比较。羊泰勒菌基因组包含一个大小为 1,638,559 bp 的单一圆形染色体，GC 含量为 38.3%，包含 1,534 个编码序列（CDS）。虽然 212 个 CDS 是羊泰勒菌特有的，但 1,322 个在马泰勒菌中有同源物。234 个马泰勒菌 CDS 在羊泰勒菌中没有同源物。对两种泰勒菌基本营养代谢的分析表明，苹果酸、谷氨酸和α-酮戊二酸可能是它们的主要碳源和能源。对于这两个物种，我们鉴定了四个不同的分泌系统和几种可能参与结合和定植宿主细胞的蛋白质，这表明它们与宿主之间有很强的相互作用潜力。与羊泰勒菌相比，马泰勒菌在毒力方面似乎装备得更好，因为我们鉴定了许多可能参与致病性的蛋白质，包括与血凝素相关的蛋白质、一个 IV 型分泌系统、TonB 依赖性乳铁蛋白和转铁蛋白受体以及 YadA 和 Hep_Hag 结构域含有的蛋白质。这是对泰勒菌属成员的首次分子特征描述，也是首次鉴定可能参与羊泰勒菌和马泰勒菌致病性和宿主定植的因素。这项研究促进了对生长表型、动物宿主偏好和致病能力的遗传理解，为未来对这个大部分未知属的功能研究铺平了道路。
