对从南非马匹的生殖道拭子和冷冻精液样本中获得的[具体内容缺失]进行直接的非培养序列分型。
Direct culture-independent sequence typing of obtained from genital swabs and frozen semen samples from South African horses.
作者信息
May Catherine E, Guthrie Alan J, Schulman Martin L
机构信息
Section of Reproduction, Department of Production Animal Studies (May, Schulman), Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, Gauteng, Republic of South Africa.
Equine Research Centre (Guthrie), Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, Gauteng, Republic of South Africa.
出版信息
J Vet Diagn Invest. 2019 Sep;31(5):792-794. doi: 10.1177/1040638719871089. Epub 2019 Aug 17.
Abstract
We report herein the use of crude extracts obtained from samples of -infected horses for the purpose of multi-locus sequence typing (MLST). Samples ( = 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at -20°C for 2-3 y) and 2 from cryopreserved raw semen aliquots (stored at -70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.
摘要
我们在此报告，为进行多位点序列分型（MLST），使用了从感染马的样本中获得的粗提物。1996年至2017年期间从南非的马匹中采集了样本（n = 36）：34份来自生殖器拭子（在-20°C下保存2 - 3年），2份来自冷冻保存的原精液等分试样（在-70°C下保存18年），然后进行检测。MLST检测显示了单一的序列类型（ST），命名为ST4，这支持了一个单点引入，因此是南非传染性马子宫炎爆发的共同来源。
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