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HAB-1，一种用于连续生产人单克隆抗体的新型异源骨髓瘤。

HAB-1, a new heteromyeloma for continuous production of human monoclonal antibodies.

作者信息

Faller G, Vollmers H P, Weiglein I, Marx A, Zink C, Pfaff M, Müller-Hermelink H K

机构信息

Institut für Pathologie, Universität Würzburg, FR Germany.

出版信息

Br J Cancer. 1990 Oct;62(4):595-8. doi: 10.1038/bjc.1990.336.

DOI:10.1038/bjc.1990.336
PMID:2223577
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1971492/
Abstract

To obtain suitable cell lines for the immortalisation of human lymphocytes, we constructed a heteromyeloma between the murine myeloma Ag8 and human lymphocytes from a highly malignant polymorphic, centroblastic B-cell lymphoma. The thioguanine-resistant and HAT-sensitive heteromyeloma HAB-1 neither secretes nor contains cytoplasmatic immunoglobulins, the cells being EBV negative but positively stained for HLA-BC and the human proliferation marker Ki-67. The karyotype consists of about 50 murine and 20 human chromosomes. The HAB-1 cells grow in suspension and have a doubling rate of about 25-30 h. In fusion experiments with spleen cells from stomach carcinoma patients HAB-1 cells show a 5-7 times higher fusion efficiency than murine Ag8 cells or another heteromyeloma SPM4-0 and give stable antibody producing products. The cell line will be made available to interested scientists.

摘要

为了获得用于人淋巴细胞永生化的合适细胞系，我们构建了一种鼠骨髓瘤Ag8与来自高度恶性多形性中心母细胞性B细胞淋巴瘤的人淋巴细胞之间的杂种骨髓瘤。抗硫鸟嘌呤且对HAT敏感的杂种骨髓瘤HAB-1既不分泌也不含有细胞质免疫球蛋白，细胞EBV阴性，但HLA-BC和人增殖标志物Ki-67呈阳性染色。核型由约50条鼠染色体和20条人染色体组成。HAB-1细胞悬浮生长，倍增时间约为25 - 30小时。在与胃癌患者脾细胞的融合实验中，HAB-1细胞显示出比鼠Ag8细胞或另一种杂种骨髓瘤SPM4-0高5 - 7倍的融合效率，并产生稳定的抗体产生产物。该细胞系将提供给感兴趣的科学家。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f60/1971492/1ef1074b704a/brjcancer00218-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f60/1971492/8c9f97e08ed1/brjcancer00218-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f60/1971492/1ef1074b704a/brjcancer00218-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f60/1971492/8c9f97e08ed1/brjcancer00218-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f60/1971492/1ef1074b704a/brjcancer00218-0049-b.jpg

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Human monoclonal autoantibodies that react with multiple endocrine organs.与多个内分泌器官发生反应的人源单克隆自身抗体。
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UC 729-6, a human lymphoblastoid B-cell line useful for generating antibody-secreting human-human hybridomas.UC 729-6，一种可用于产生分泌抗体的人-人杂交瘤的人淋巴母细胞样B细胞系。
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Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production.用于生产人单克隆抗体的小鼠-人异种骨髓瘤的构建与测试。
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