Oncology Unit, Animal Health Trust, Newmarket, UK.
Vet Comp Oncol. 2012 Jun;10(2):135-42. doi: 10.1111/j.1476-5829.2011.00287.x. Epub 2011 Sep 5.
Minichromosome maintenance proteins (MCMs) are sensitive markers of cellular proliferation and have been shown to be significant predictors of survival in several human malignancies. MCM7 was evaluated as a prognostic marker in canine cutaneous mast cell tumours (MCTs). MCM7 immunohistochemistry was performed and an index of MCM7-positive cells calculated in dogs with known outcome. The Receiver Operating Characteristics method was used to individuate the best cut-off value of MCM7 score as predictor of survival. Survival analysis and prognostic variables were analysed with statistical methods. Ninety-five dogs were included with 31 dying of MCTs. A value of 0.18 was used as cut-off value of MCM7 score as a binary variable. The median survival time for MCM7 score ≤0.18 was not reached at 3668 days, whereas for MCM7 score >0.18 was 187 days (log-rank test; P < 0.0001). In the multivariable analysis, MCM7 was significantly associated with survival after controlling for age, surgical margins and histological grade (hazard ratio 9.2; P = 0.001).
微染色体维持蛋白（MCMs）是细胞增殖的敏感标志物，已被证明是几种人类恶性肿瘤生存的重要预测因子。MCM7 被评估为犬皮肤肥大细胞瘤（MCTs）的预后标志物。对已知结局的犬进行 MCM7 免疫组化，并计算 MCM7 阳性细胞指数。采用受试者工作特征（ROC）曲线法确定 MCM7 评分作为生存预测的最佳截断值。采用统计学方法分析生存分析和预后变量。共纳入 95 例犬，其中 31 例死于 MCTs。将 MCM7 评分≤0.18 定义为二分类变量的截断值为 0.18。MCM7 评分≤0.18 的中位生存时间未达到 3668 天，而 MCM7 评分>0.18 的中位生存时间为 187 天（对数秩检验；P<0.0001）。在多变量分析中，在控制年龄、手术切缘和组织学分级后，MCM7 与生存显著相关（风险比 9.2；P=0.001）。
