Masuyama Kaoru, Zhang Yi, Rao Yi, Wang Jing W
Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California , USA.
J Neurogenet. 2012 Mar;26(1):89-102. doi: 10.3109/01677063.2011.642910. Epub 2012 Jan 12.
Abstract: Nuclear factor of activated T cells (NFAT) is a calcium-responsive transcription factor. We describe here an NFAT-based neural tracing method-CaLexA (calcium-dependent nuclear import of LexA)-for labeling active neurons in behaving animals. In this system, sustained neural activity induces nuclear import of the chimeric transcription factor LexA-VP16-NFAT, which in turn drives green fluorescent protein (GFP) reporter expression only in active neurons. We tested this system in Drosophila and found that volatile sex pheromones excite specific neurons in the olfactory circuit. Furthermore, complex courtship behavior associated with multi-modal sensory inputs activated neurons in the ventral nerve cord. This method harnessing the mechanism of activity-dependent nuclear import of a transcription factor can be used to identify active neurons in specific neuronal population in behaving animals.
活化T细胞核因子（NFAT）是一种钙反应性转录因子。我们在此描述一种基于NFAT的神经追踪方法——CaLexA（LexA的钙依赖性核输入），用于标记行为动物中的活跃神经元。在该系统中，持续的神经活动诱导嵌合转录因子LexA-VP16-NFAT的核输入，进而仅在活跃神经元中驱动绿色荧光蛋白（GFP）报告基因的表达。我们在果蝇中测试了该系统，发现挥发性性信息素可激发嗅觉回路中的特定神经元。此外，与多模式感觉输入相关的复杂求偶行为激活了腹神经索中的神经元。这种利用转录因子活性依赖性核输入机制的方法可用于识别行为动物中特定神经元群体中的活跃神经元。
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