Liu Yue, Xie Ming, Zhang Ye
China Academy of Chinese Medical Sciences, Beijing, China.
Zhong Xi Yi Jie He Xue Bao. 2012 Jan;10(1):100-8. doi: 10.3736/jcim20120115.
To observe the development of Chinese medicine syndromes of rats with type 2 diabetes and insulin resistance by detecting dynamic changes of laboratory parameters.
A total of 110 male Sprague-Dawley rats were randomly divided into control group (n=50) and model group (n=60) to observe the correlation between laboratory parameter changes and development of Chinese medicine syndromes. A rat model of type 2 diabetes and insulin resistance was established by feeding rats with high-calorie and high-fat diet together with peritoneal injection of streptozocin (30 mg/kg, once). At the end of 0, 6, 8, 10 and 12 weeks, 10 rats were randomly selected form each group and blood was taken from common carotid artery to detect contents of total cholesterol (TC), triacylglycerol (TAG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and leptin in serum, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) contents in plasma and whole blood and plasma viscosities.
Compared with the control group, concentrations of TNF-α, IL-6, leptin and cAMP and ratio of cAMP/cGMP increased and content of cGMP decreased markedly from the 6th week to the 12th week. Concentrations of TC, TAG and LDL-C increased from the 8th week to the 12th week, while HDL-C decreased from the 10th week to the 12th week. Whole blood and plasma viscosity values under different shear rates increased significantly from the 10th week to the 12th week.
Based on the understanding of markers which have close relationship with Chinese medicine syndromes of dual deficiency of qi and yin, turbid phlegm and blood stasis, combining with the dynamic changes of appearance behavior and laboratory parameters of this animal experiment, the authors speculate the development characteristics of Chinese medicine syndromes in rats with type 2 diabetes. The dynamic progress of Chinese medicine syndromes and pathology varies from dual deficiency of qi and yin to dual deficiency of qi and yin with turbid phlegm, and at last to the combination of dual deficiency of qi and yin, turbid phlegm and blood stasis.
通过检测实验室指标的动态变化，观察2型糖尿病合并胰岛素抵抗大鼠中医证候的演变。
将110只雄性Sprague-Dawley大鼠随机分为对照组（n = 50）和模型组（n = 60），观察实验室指标变化与中医证候演变的相关性。采用高热量高脂肪饮食联合腹腔注射链脲佐菌素（30 mg/kg，一次性）建立2型糖尿病合并胰岛素抵抗大鼠模型。在0、6、8、10和12周末，每组随机选取10只大鼠，经颈总动脉取血，检测血清总胆固醇（TC）、三酰甘油（TAG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和瘦素含量，血浆和全血中环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）含量及全血和血浆黏度。
与对照组比较，从第6周开始至第12周，模型组大鼠血清TNF-α、IL-6、瘦素及cAMP含量和cAMP/cGMP比值明显升高，cGMP含量明显降低；从第8周开始至第12周，TC、TAG和LDL-C浓度升高，HDL-C从第10周开始至第12周降低；从第10周开始至第12周，不同切变率下的全血和血浆黏度值显著升高。
基于对气阴两虚、痰浊血瘀中医证候相关指标的认识，结合本动物实验外观行为和实验室指标的动态变化，推测2型糖尿病大鼠中医证候的演变特点。中医证候与病理的动态进展为从气阴两虚证发展为气阴两虚兼痰浊证，最终发展为气阴两虚、痰浊血瘀兼夹证。
