Mohan Maruga Raja Muthu Kumaradoss, Mishra Shri Hari
Department of Pharmacognosy and Phytochemistry, Karnataka Kaveri Educational and Cultural Society College of Pharmacy, Bangalore, Karnataka, India.
Zhong Xi Yi Jie He Xue Bao. 2012 Jan;10(1):109-13. doi: 10.3736/jcim20120116.
Clerosterol palmityl ester (CPE) is a unique clerosterol derivative isolated and characterized from the leaves of Clerodendrum phlomidis. Considering the uniqueness of this compound, the present study was planned to use CPE as a specific chemical marker and develop a new validated thin-layer chromatography (TLC) method for standardisation of C. phlomidis.
Separation and quantification of CPE were achieved by TLC using a mobile phase of petroleum ether (60 to 80 degrees centigrade) and ethyl acetate (95:5, volume ratio) (Rf 0.64) on precoated silica gel 60F(254) aluminium plates. Densitometric determination was carried out after derivatization with anisaldehyde sulphuric acid reagent in absorption mode at 527 nm.
The calibration curve was linear in the concentration range of 100 to 500 ng/spot. The method was validated for precision, repeatability and accuracy. The proposed method was found to be simple, specific, precise, accurate, rapid and cost-effective.
This TLC procedure may be used effectively for quantitative determination of CPE, identification of the plant and standardization of this plant or its derived products.
clerosterol棕榈酸酯（CPE）是从臭牡丹叶中分离并鉴定出的一种独特的clerosterol衍生物。鉴于该化合物的独特性，本研究计划将CPE用作特定化学标志物，并开发一种经过验证的新型薄层色谱（TLC）方法用于臭牡丹的标准化。
采用薄层色谱法，以石油醚（60至80摄氏度）和乙酸乙酯（体积比95:5）作为流动相，在预涂硅胶60F(254)铝板上对CPE进行分离和定量（比移值0.64）。用茴香醛硫酸试剂衍生化后，于527nm波长处采用吸收模式进行光密度测定。
校准曲线在100至500ng/斑点的浓度范围内呈线性。该方法在精密度、重复性和准确性方面得到了验证。结果表明，所提出的方法简单、特异、精密、准确、快速且具有成本效益。
该薄层色谱法可有效用于CPE的定量测定、植物鉴定以及该植物或其衍生产品的标准化。
