建立一个古DNA实验室。

Setting up an ancient DNA laboratory.

作者信息

Fulton Tara L

机构信息

Department of Biology, The Pennsylvania State University, 320 Mueller Laboratory, University Park, PA 16802, USA.

出版信息

Methods Mol Biol. 2012;840:1-11. doi: 10.1007/978-1-61779-516-9_1.

DOI:10.1007/978-1-61779-516-9_1

PMID:22237515

Abstract

Entering into the world of ancient DNA research is nontrivial. Because the DNA in most ancient specimens is degraded to some extent, the potential for contamination of ancient samples and DNA extracts with modern DNA is considerable. To minimize the risk associated with working with ancient DNA, experimental protocols specific to handling ancient specimens have been introduced. Here, I outline the challenges associated with working with ancient DNA and describe guidelines for setting up a new ancient DNA laboratory. I also discuss steps that can be taken at the sample collection and preparation stage to minimize the potential for contamination with exogenous sources of DNA.

摘要

进入古代DNA研究领域并非易事。由于大多数古代样本中的DNA都有一定程度的降解，古代样本和DNA提取物被现代DNA污染的可能性相当大。为了将与古代DNA研究相关的风险降至最低，已经引入了处理古代样本的特定实验方案。在此，我概述了与古代DNA研究相关的挑战，并描述了建立一个新的古代DNA实验室的指导方针。我还讨论了在样本采集和制备阶段可以采取的措施，以尽量减少外源DNA污染的可能性。