Barnett Ross, Larson Greger
Department of Archaeology, Durham University, South Road, Durham, DH1 3LE, UK.
Methods Mol Biol. 2012;840:13-9. doi: 10.1007/978-1-61779-516-9_2.
The myriad downstream applications of ancient DNA (aDNA) analysis all ultimately require that sequence data are generated from extracts of ancient material. DNA extraction from tissues known to contain preserved biomolecules (e.g. teeth, hair, tissue, bone) relies on subtle modifications of a basic technique that has been in use for nearly two decades. Multiple DNA extraction protocols have been introduced, with varying levels of success depending on tissue type and the long-term preservation environment to which the ancient tissue was exposed. Here, we describe the phenol-chloroform method for extracting aDNA from any tissue type. This commonly employed method allows for the recovery of total nucleic acid content with minimal loss of low molecular weight double-stranded DNA.
古代DNA（aDNA）分析的众多下游应用最终都要求从古代材料提取物中生成序列数据。从已知含有保存生物分子的组织（如牙齿、毛发、组织、骨骼）中提取DNA依赖于一种已使用近二十年的基本技术的细微改进。已经引入了多种DNA提取方案，其成功程度因组织类型和古代组织所处的长期保存环境而异。在这里，我们描述了从任何组织类型中提取aDNA的酚-氯仿法。这种常用方法能够以最少的低分子量双链DNA损失回收总核酸含量。
