Ibikunle Gabriel Femi, Adebajo Adeleke Clement, Famuyiwa Funmilayo Gladys, Aladesanmi Adetunji Joseph, Adewunmi Clement Oladapo
Department of Pharmacognosy Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
Afr J Tradit Complement Altern Med. 2011;8(2):170-6. Epub 2010 Dec 30.
Eugenia uniflora, used ethnomedically in some tropical countries as an anti-infective, has shown anti-malarial and anti-trypanocidal activities. Therefore using bioactivity guided fractionation, anti-trichomonal activity of E. uniflora leaf was investigated. Anti-trichomonal activities of leaf methanol extract and its fractions against Trichomonas gallinae as well as their cytotoxicities using an in vitro haemaglutination assay were determined. Anti-trichomonacidal activities of the extract improved on purification up to a stage. Subfractions E(2-5) had LC(50) and LC(90) values of 4.77 - 5.28, 18.49 - 25.00 and 4.53 - 5.18, 18.32 - 19.07 µg/ml at 24 and 48 hrs, respectively that were better than those of metronidazole. Further purification of E(2-5) led to loss of activity suggesting that the active components were probably working synergistically and additively. Demonstration of low haemaglutination titre values of 0.00 - 5.33 by methanolic extract and its partition fractions suggested their low toxicity profile. The established safety of the leaf indicated that its anti-trichomonal activity was not due to non-specific cytotoxicity, hence could be used in ethnomedicine as an anti-trichomonal agent.
番樱桃在一些热带国家被用作抗感染的民间药物，已显示出抗疟疾和抗锥虫活性。因此，采用生物活性导向分级分离法，对番樱桃叶的抗滴虫活性进行了研究。使用体外血凝试验测定了叶甲醇提取物及其馏分对鸡三毛滴虫的抗滴虫活性及其细胞毒性。提取物的抗滴虫活性在纯化到一定阶段后有所提高。亚馏分E(2-5)在24小时和48小时时的LC(50)和LC(90)值分别为4.77 - 5.28、18.49 - 25.00和4.53 - 5.18、18.32 - 19.07µg/ml，优于甲硝唑。对E(2-5)进一步纯化导致活性丧失，这表明活性成分可能具有协同和相加作用。甲醇提取物及其分配馏分的血凝滴度值较低，为0.00 - 5.33，表明其毒性较低。该叶已确定的安全性表明其抗滴虫活性并非由于非特异性细胞毒性，因此可在民族医学中用作抗滴虫剂。
