National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China.
Biotechnol Prog. 2011 Nov-Dec;27(6):1661-71. doi: 10.1002/btpr.682.
A two-dimensional axisymmetric computational fluid dynamics (CFD) model based on a porous media model and a discrete population balance model was established to investigate the hydrodynamics and mass transfer behavior in an airlift bioreactor for hairy root culture.During the hairy root culture of Echinacea purpurea, liquid and gas velocity, gas holdup, mass transfer rate, as well as oxygen concentration distribution in the airlift bioreactor were simulated by this CFD model. Simulative results indicated that liquid flow and turbulence played a dominant role in oxygen mass transfer in the growth domain of the hairy root culture. The dissolved oxygen concentration in the hairy root clump increased from the bottom to the top of the bioreactor cultured with the hairy roots, which was verified by the experimental detection of dissolved oxygen concentration in the hairy root clump. This methodology provided insight understanding on the complex system of hairy root culture and will help to eventually guide the bioreactor design and process intensification of large-scale hairy root culture.
建立了基于多孔介质模型和离散种群平衡模型的二维轴对称计算流体动力学（CFD）模型，以研究毛状根培养中气升式生物反应器中的流体动力学和传质行为。在紫锥菊毛状根培养过程中，通过该 CFD 模型模拟了气升式生物反应器中的液体和气体速度、气体含率、传质速率以及氧气浓度分布。模拟结果表明，在毛状根培养的生长区域中，液体流动和湍流对氧气传质起主导作用。用毛状根培养的生物反应器中，毛状根丛底部到顶部的溶解氧浓度逐渐增加，这通过毛状根丛中溶解氧浓度的实验检测得到了验证。该方法为毛状根培养这一复杂体系提供了深入的认识，并将有助于最终指导生物反应器设计和大规模毛状根培养的过程强化。
