National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
Adv Biochem Eng Biotechnol. 2013;134:91-114. doi: 10.1007/10_2013_181.
The cultivation of hairy roots for the production of secondary metabolites offers numerous advantages; hairy roots have a fast growth rate, are genetically stable, and are relatively simple to maintain in phytohormone free media. Hairy roots provide a continuous source of secondary metabolites, and are useful for the production of chemicals for pharmaceuticals, cosmetics, and food additives. In order for hairy roots to be utilized on a commercial scale, it is necessary to scale-up their production. Over the last several decades, significant research has been conducted on the cultivation of hairy roots in various types of bioreactor systems. In this review, we discuss the advantages and disadvantages of various bioreactor systems, the major factors related to large-scale bioreactor cultures, process intensification technologies and overview the mathematical models and computer-aided methods that have been utilized for bioreactor design and development.
毛状根培养用于生产次生代谢产物具有许多优点；毛状根生长速度快、遗传稳定，在无植物激素的培养基中相对容易维持。毛状根提供了次生代谢产物的持续来源，可用于生产医药、化妆品和食品添加剂用化学品。为了在商业规模上利用毛状根，有必要扩大其生产。在过去几十年中，人们在各种类型的生物反应器系统中对毛状根的培养进行了大量研究。在这篇综述中，我们讨论了各种生物反应器系统的优缺点、与大规模生物反应器培养相关的主要因素、强化技术，并概述了用于生物反应器设计和开发的数学模型和计算机辅助方法。
