State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.
Biointerphases. 2011 Dec;6(4):143-52. doi: 10.1116/1.3644381.
Cellular micropatterning with bio-adhesive and nonadhesive areas has attracted increasing interest for the precise design of cell-to-surface attachment in cell biology studies, tissue engineering, cell-based biosensors, biological assays, and drug development and screening. In this paper we describe a simple and efficient method to create a two-dimensional stable cellular microenvironment, which is based on (1) forming a protein-resistant oligo(ethylene glycol) methyl ether methacrylate polymer layer on the substrates via surface-initiated atom transfer radical polymerization; (2) placing a defined photomask on the substrate and exposing the substrate to ultraviolet light; and (3) immersing the patterned surface in a fibronectin solution to form cell-adhesive protein patterns in a cell-resistant background. The resulting surfaces are tailored into cell-adhesive and cell-resistant regions. Three different types of cells (NIH-3T3, PC12, bone marrow-derived mesenchymal stem cells) are seeded on such patterned surfaces to form cellular patterns. The geometric effects on cell behavior are investigated. The long-term stability is tested by NIH-3T3 fibroblasts and mesenchymal stem cells and excellent retention of cellular patterns is observed. The strategy illustrated here offers an efficient way to create a stable, patterned cellular microenvironment, and could be employed in tissue engineering to study the effect of micropatterns on the proliferation and differentiation of cells, and in particular mesenchymal stem cells.
细胞与表面黏附的精确设计在细胞生物学研究、组织工程、基于细胞的生物传感器、生物测定、药物开发和筛选中受到越来越多的关注。在本文中，我们描述了一种简单有效的方法来创建二维稳定的细胞微环境，该方法基于以下三个步骤：(1) 通过表面引发原子转移自由基聚合在基底上形成抗蛋白的聚乙二醇甲基醚甲基丙烯酸酯聚合物层；(2) 将定义好的光掩模放在基底上，并使基底暴露于紫外线下；(3) 将图案化的表面浸入纤维连接蛋白溶液中，在细胞抗性背景下形成细胞黏附蛋白图案。得到的表面被定制成细胞黏附区和细胞抵抗区。将三种不同类型的细胞（NIH-3T3、PC12、骨髓间充质干细胞）接种到这种图案化的表面上以形成细胞图案。研究了细胞行为的几何效应。通过 NIH-3T3 成纤维细胞和间充质干细胞对其长期稳定性进行了测试，观察到细胞图案的优异保留。这里所示的策略提供了一种创建稳定的、图案化的细胞微环境的有效方法，可用于组织工程研究微图案对细胞增殖和分化的影响，特别是间充质干细胞。
