Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Langmuir. 2011 May 17;27(10):6155-62. doi: 10.1021/la200487w. Epub 2011 Apr 12.
Micropatterned surfaces are very useful to control cell microenvironment and investigate the physical effects on cell function. In this study, poly(vinyl alcohol) (PVA) micropatterns on polystyrene cell-culture plates were prepared using UV photolithography. Cell adhesive polystyrene geometries of triangle, square, pentagon, hexagon, and circle were surrounded by cell nonadhesive PVA to manipulate cell shapes. These different geometries had the same small surface areas for cell spreading. Human mesenchymal stem cells (MSCs) were cultured on the micropatterned surface, and the effect of cell geometry on adipogenic differentiation was investigated. MSCs adhered to the geometric micropatterns and formed arrays of single cell with different shapes. The distribution patterns of actin filaments were similar among these cell shapes and remolded during adipogenesis. The adipogenic differentiation potential of MSCs was similar on the small size triangular, square, pentagonal, hexagonal, and circular geometries according to lipid vacuoles staining. This simple micropatterning technique using photoreactive molecules will be useful for creating micropatterns of arbitrary design on an organic surface, and cell functions can be directly and systematically compared on a single surface without external factors resulting from separate cell culture and coating method.
微图案表面对于控制细胞微环境和研究物理效应对细胞功能的影响非常有用。在这项研究中，使用紫外光刻技术在聚苯乙烯细胞培养板上制备了聚乙烯醇（PVA）微图案。用不具有细胞黏附性的 PVA 将具有细胞黏附性的三角形、正方形、五边形、六边形和圆形聚苯乙烯几何图形包围起来，以操纵细胞形状。这些不同的几何图形具有相同的用于细胞扩展的小表面积。将人骨髓间充质干细胞（MSCs）培养在微图案表面上，研究了细胞形状对成脂分化的影响。MSCs 黏附在几何微图案上，并形成具有不同形状的单细胞阵列。在这些细胞形状中，肌动蛋白丝的分布模式相似，并在成脂分化过程中重塑。根据脂滴染色，MSCs 在小尺寸的三角形、正方形、五边形、六边形和圆形几何图形上的成脂分化潜力相似。这种使用光反应分子的简单微图案化技术将有助于在有机表面上创建任意设计的微图案，并且可以在单个表面上直接和系统地比较细胞功能，而不会受到由于单独的细胞培养和涂层方法而导致的外部因素的影响。
