Inserm U957, Bone Resorption Physiopathology and Primary Bone Tumor Therapy, Faculty of Medicine, University of Nantes, Nantes, France.
Acta Biomater. 2011 Apr;7(4):1525-34. doi: 10.1016/j.actbio.2010.12.033. Epub 2010 Dec 31.
Understanding of the interactions between cells and surfaces is essential in the field of tissue engineering and biomaterials. This study aimed to compare the adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs), an osteoblast cell line (MC3T3-E1) and gingival fibroblasts (HGF-1) on tissue culture polystyrene (TCPS), glass and titanium (Ti). The average surface roughness was 5, 0.2 and 40×10(-3) μm for TCPS, glass and Ti, respectively. Immunocytochemistry and image analysis made it possible to quantify the number and morphology of adherent cells as well as the density of the focal points. Regardless of the substrate, both hMSCs and osteoblastic cells were mainly branch-shaped. HGF-1 exhibited a significantly higher number of focal points on Ti than on TCPS and glass. Alizarin red quantification indicated that both hMSCs and osteoblastic cells were more differentiated on TCPS than on Ti and glass. The surface properties of substrates, such as roughness, wettability and chemical composition, modulated the behaviour of the cells. Early events, such as cell adhesion, may influence the differentiation of hMSC and consequently tissue healing around implanted biomaterials.
理解细胞与表面之间的相互作用是组织工程和生物材料领域的关键。本研究旨在比较人骨髓间充质干细胞（hMSC）、成骨细胞系（MC3T3-E1）和牙龈成纤维细胞（HGF-1）在组织培养聚苯乙烯（TCPS）、玻璃和钛（Ti）表面的黏附、增殖和分化情况。TCPS、玻璃和 Ti 的平均表面粗糙度分别为 5、0.2 和 40×10(-3)μm。免疫细胞化学和图像分析可定量黏附细胞的数量和形态以及焦点密度。无论使用哪种基质，hMSC 和成骨细胞均主要呈分支状。HGF-1 在 Ti 上的焦点数量明显多于 TCPS 和玻璃。茜素红定量分析表明，hMSC 和成骨细胞在 TCPS 上的分化程度高于 Ti 和玻璃。基底的表面特性，如粗糙度、润湿性和化学成分，可调节细胞的行为。早期事件，如细胞黏附，可能影响 hMSC 的分化，进而影响植入生物材料周围组织的愈合。
