Clinical and Experimental Optometry Research Lab, Center of Physics (Optometry), University of Minho, Braga, Portugal.
Cornea. 2012 Jun;31(6):633-8. doi: 10.1097/ICO.0b013e31823f0905.
To show the use of high-resolution spectral-domain optical coherence tomography (HR-SOCT) for the in situ evaluation of epithelial, stromal, and contact lens (CL) thickness changes under closed-eye conditions without lens removal.
Eight young healthy patients wore a thick soft CL for 90 minutes under closed-eye conditions, and measures of epithelial and stromal corneal thickness were obtained at regular intervals using a HR-SOCT (Copernicus HR; Optopol Tech. SA, Zawiercie, Poland).
Minimal changes in epithelial thickness were detected with a transient statistically significant increase in epithelial thickness in the fellow control eye 30 minutes after insertion (P = 0.028). A significant and progressive increase in stromal thickness up to 8% after 90 minutes of lens wear was observed at a constant rate of 2.5% every 30 minutes, being statistically significant in all observations (P < 0.001). Fellow control eyes also showed a significant increase in stromal thickness at a much lower rate of 0.5% every 30 minutes. Lens thickness decreased significantly by 2% after 90 minutes of lens wear under closed-eye conditions (P < 0.001). Individual analysis showed that all eyes displayed stromal swelling, whereas only half of them showed epithelial swelling.
Increase in stromal thickness and a slight decrease in lens thickness were observed in response to a hypoxic stimulus under closed-eye conditions. HR-SOCT is a powerful tool to investigate in vivo the physiological interactions between cornea and CLs.
展示在不取出镜片的闭眼下条件下，使用高分辨率谱域光相干断层扫描（HR-SOCT）原位评估上皮、基质和隐形眼镜（CL）厚度变化的用途。
8 名年轻健康的患者在闭眼下条件下佩戴厚的软性 CL 90 分钟，每隔一段时间使用 HR-SOCT（Copernicus HR；Optopol Tech. SA，Zawiercie，波兰）测量上皮和基质角膜厚度。
仅检测到上皮厚度的微小变化，在插入后 30 分钟，对侧对照眼的上皮厚度出现短暂的统计学显著增加（P = 0.028）。在 90 分钟的镜片佩戴过程中，观察到基质厚度以 2.5%/30 分钟的恒定速率显著且渐进性增加，直至增加 8%，所有观察均具有统计学意义（P < 0.001）。对侧对照眼的基质厚度也以 0.5%/30 分钟的低得多的速度显著增加。在闭眼下条件下，镜片佩戴 90 分钟后，镜片厚度显著减少 2%（P < 0.001）。个体分析显示，所有眼睛均显示基质肿胀，而只有一半眼睛显示上皮肿胀。
在闭眼下缺氧刺激下，观察到基质厚度增加和镜片厚度轻微减少。HR-SOCT 是一种强大的工具，可用于研究角膜和隐形眼镜之间的生理相互作用。
