Grupo de Microbiología Molecular, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia.
Mem Inst Oswaldo Cruz. 2011 Dec;106(8):1017-23. doi: 10.1590/s0074-02762011000800020.
To elucidate the Anopheles nuneztovari s.l. taxonomic status at a microgeographic level in four malaria endemic localities from Antioquia and Córdoba, Colombia, fragments of the cytochrome oxidase subunit I (COI) and the white gene were used. The COI analysis showed low genetic differentiation with fixation index (F(ST)) levels between -0.02-0.137 and Nm values between 3-∞, indicating the presence of high gene flow among An. nuneztovari s.l. populations from the four localities. The COI network showed a single most common haplotype, type 1 (n = 55), present in all localities, as the likely ancestral haplotype. Analysis of the white gene showed that An. nuneztovari s.l. populations from both departments grouped with haplotypes 19 and 20, which are part of lineage 3 reported previously. The results of the present study suggest that An. nuneztovari s.l. is a single taxon in the area of the present study.
为了阐明在哥伦比亚安蒂奥基亚省和科尔多瓦省的四个疟疾流行地区的微地理水平上的按蚊努涅斯亚种的分类地位，我们使用了细胞色素氧化酶亚基 I（COI）和白色基因片段。COI 分析显示遗传分化程度较低，固定指数（F（ST））水平在-0.02 到 0.137 之间，Nm 值在 3 到无穷大之间，表明四个地方的按蚊努涅斯亚种群体之间存在高度的基因流动。COI 网络显示了一个单一的最常见的单倍型，类型 1（n = 55），存在于所有的地方，作为可能的祖先单倍型。白色基因分析表明，来自两个省的按蚊努涅斯亚种群体与先前报道的第 3 谱系中的单倍型 19 和 20 聚在一起。本研究的结果表明，在本研究的区域内，按蚊努涅斯亚种是一个单一的分类单元。
