Horn R A, Friesen E J, Stephens R L, Hedrick W R, Zimbrick J D
Cancer. 1979 Jun;43(6):2392-8. doi: 10.1002/1097-0142(197906)43:6<2392::aid-cncr2820430633>3.0.co;2-g.
The methodology of blood sample preparation and analysis has been examined to further evaluate the technique of electron spin resonance (ESR) for possible use in detecting cancer and in monitoring the progress of cancer therapy. Frozen whole blood and serum samples from 278 normal donors and 97 cancer patients were studied by ESR for signal intensity from Cu+2 bound to ceruloplasmin (g factor = 2.05). The signal from this species (Cu+2-CP) in serum rose sharply during the first two hours of storage at room temperature after being drawn from the subject, and then reached a plateau. The average Cu+2-CP ESR signal intensity was significantly different for control groups of males, females not taking estrogen medication, and females taking estrogens. The mean ESR signal intensities of Cu+2-CP from cancer patients separated into the same groups as the control data were approximately twice as great as the mean control levels. Total serum copper levels were correlated with ESR intensities of Cu+2-CP and indicated that the ratio of Cu+2/Cu+1 in CP is higher in serum from cancer patients than from controls.
对血样制备和分析方法进行了研究，以进一步评估电子自旋共振（ESR）技术在检测癌症及监测癌症治疗进展方面的潜在用途。通过ESR研究了278名正常献血者和97名癌症患者的冷冻全血及血清样本中与铜蓝蛋白结合的Cu+2（g因子 = 2.05）的信号强度。从受试者体内抽取的血清中，该物质（Cu+2-CP）的信号在室温下储存的前两小时内急剧上升，然后达到平稳状态。男性对照组、未服用雌激素药物的女性对照组以及服用雌激素的女性对照组的平均Cu+2-CP ESR信号强度存在显著差异。与对照数据分为相同组别的癌症患者的Cu+2-CP平均ESR信号强度约为对照水平的两倍。血清总铜水平与Cu+2-CP的ESR强度相关，表明癌症患者血清中铜蓝蛋白的Cu+2/Cu+1比值高于对照组。
