Department of Chemical Engineering, University of Castilla-La Mancha, Ciudad Real, Spain.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(2):274-9. doi: 10.1080/10934529.2012.640906.
The mobilisation of diesel-degrading microorganisms in soils of three different textures (sandy, clay and silty) using electrokinetic techniques was studied. The mobilisation tests were performed using a laboratory-scale electrokinetic cell in which a synthetic soil column was inserted between the cathode and anode compartments. Microorganisms were located at the anode compartment at the beginning of each assay. A constant cell voltage was applied, and samples were taken from the cathode and anode compartments. Microbial transport through the soil strongly depended on soil particle size. Small particle sizes (silty and clay soil) travelled at low velocities (microbial transport rates of approximately 0.06 and 0.17 cm/min, respectively), while large particle sizes (sandy soil) led to high numbers of microorganisms passing through the soil column. In sandy soil, an increase in the voltage gradient did not increase the quantity of mobilised microorganisms (approximately 10(7) CFU/mL for every voltage gradient applied). For clay and silty soils, a higher voltage gradient led to a higher quantity of microorganisms mobilised to the cathodic compartment and a lower delay time for detecting the presence of microorganisms in the same compartment.
采用电动技术研究了三种不同质地（沙质、粘质和粉质）土壤中柴油降解微生物的迁移情况。在实验室规模的电动电池中进行了迁移测试，在阴极和阳极室之间插入了一个合成土壤柱。在每次测定开始时，微生物都位于阳极室。施加恒定的电池电压，并从阴极和阳极室取样。微生物在土壤中的迁移强烈依赖于土壤粒径。小粒径（粉质和粘质土壤）迁移速度较低（微生物迁移率分别约为 0.06 和 0.17 cm/min），而大粒径（沙质土壤）导致大量微生物通过土壤柱。在沙质土壤中，增加电压梯度不会增加迁移微生物的数量（对于施加的每个电压梯度，约为 10(7) CFU/mL）。对于粘质和粉质土壤，较高的电压梯度导致更多的微生物被迁移到阴极室，并且在同一室中检测到微生物存在的延迟时间更短。
