Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, Munich, Germany.
ACS Nano. 2012 Feb 28;6(2):1370-9. doi: 10.1021/nn204187c. Epub 2012 Jan 24.
The behavior of quantum dots (QDs) in the microvasculature and their impact on inflammatory reactions under pathophysiological conditions are still largely unknown. Therefore, we designed this study to investigate the fate and effects of surface-modified QDs in postischemic skeletal and heart muscle. Under these pathophysiological conditions, amine-modified QDs, but not carboxyl-QDs, were strongly associated with the vessel wall of postcapillary venules and amplified ischemia-reperfusion-elicited leukocyte transmigration. Importantly, strong association of amine-QDs with microvessel walls was also present in the postischemic myocardium. As shown by electron microscopy and verified by FACS analyses, amine-modified QDs, but not carboxyl-QDs, were associated with endogenous microparticles. At microvessel walls, these aggregates were attached to endothelial cells. Taken together, we found that both the surface chemistry of QDs and the underlying tissue conditions (i.e., ischemia-reperfusion) strongly determine their uptake by endothelial cells in microvessels, their association to endogenous microparticles, as well as their potential to modify inflammatory processes. Thus, this study strongly corroborates the view that the surface chemistry of nanomaterials and the physiological state of the tissue are crucial for the behavior of nanomaterials in vivo.
量子点（QDs）在微血管中的行为及其在病理生理条件下对炎症反应的影响在很大程度上尚不清楚。因此，我们设计了这项研究，以调查表面修饰的 QDs 在缺血后骨骼肌和心肌中的命运和作用。在这些病理生理条件下，胺修饰的 QDs 而不是羧基-QDs 与毛细血管后微静脉的血管壁强烈结合，并放大缺血再灌注引起的白细胞迁移。重要的是，在缺血后的心肌中也存在与微血管壁强烈结合的胺-QDs。正如电子显微镜所示，并通过 FACS 分析验证，胺修饰的 QDs 而不是羧基-QDs 与内源性微粒体结合。在微血管壁上，这些聚集物附着在内皮细胞上。总之，我们发现 QDs 的表面化学性质和基础组织条件（即缺血再灌注）强烈决定了它们在内皮细胞中的摄取、与内源性微粒体的结合以及它们修饰炎症过程的潜力。因此，这项研究强烈证实了纳米材料的表面化学性质和组织的生理状态对纳米材料在体内行为的重要性。
