Australian Centre for Microscopy & Microanalysis and The School of Biological Sciences, The University of Sydney, Sydney, NSW 2006, Australia.
Micron. 2012 Apr;43(5):565-82. doi: 10.1016/j.micron.2011.12.004. Epub 2011 Dec 23.
Correlative microscopy is the application of two or more distinct microscopy techniques to the same region of a sample, generating complementary morphological, structural and chemical information that exceeds what is possible with any single technique. As a variety of complementary microscopy approaches rather than a specific type of instrument, correlative microscopy has blossomed in recent years as researchers have recognised that it is particularly suited to address the intricate questions of the modern biological sciences. Specialised technical developments in sample preparation, imaging methods, visualisation and data analysis have also accelerated the uptake of correlative approaches. In light of these advances, this critical review takes the reader on a journey through recent developments in, and applications of, correlative microscopy, examining its impact in biomedical research and in the field of plant science. This twin emphasis gives a unique perspective into use of correlative microscopy in fields that often advance independently, and highlights the lessons that can be learned from both fields for the future of this important area of research.
相关显微镜技术是将两种或多种不同的显微镜技术应用于样品的同一区域，生成互补的形态、结构和化学信息，这些信息超出了任何单一技术所能提供的信息。作为多种互补显微镜方法而不是特定类型的仪器，相关显微镜技术近年来蓬勃发展，因为研究人员认识到它特别适合解决现代生物学科学中复杂的问题。样品制备、成像方法、可视化和数据分析方面的专门技术发展也加速了相关方法的应用。鉴于这些进展，本综述批判性地回顾了相关显微镜技术的最新发展和应用，考察了它在生物医学研究和植物科学领域的影响。这种双重重点为读者提供了在通常独立发展的领域中使用相关显微镜技术的独特视角，并强调了可以从这两个领域为这一重要研究领域的未来吸取的经验教训。
