Hubbard Center for Genome Studies, University of New Hampshire, 35 Colovos Rd, Durham, NH 03824, USA.
Trends Ecol Evol. 2012 Apr;27(4):233-43. doi: 10.1016/j.tree.2011.11.010. Epub 2012 Jan 11.
Microscopic eukaryotes are abundant, diverse and fill critical ecological roles across every ecosystem on Earth, yet there is a well-recognized gap in understanding of their global biodiversity. Fundamental advances in DNA sequencing and bioinformatics now allow accurate en masse biodiversity assessments of microscopic eukaryotes from environmental samples. Despite a promising outlook, the field of eukaryotic marker gene surveys faces significant challenges: how to generate data that are most useful to the community, especially in the face of evolving sequencing technologies and bioinformatics pipelines, and how to incorporate an expanding number of target genes.
微观真核生物丰富多样，在地球上的每个生态系统中都发挥着关键的生态作用，但人们对它们的全球生物多样性的认识仍存在明显的差距。DNA 测序和生物信息学的重大进展现在使我们能够从环境样本中对微观真核生物进行准确的大规模生物多样性评估。尽管前景广阔，但真核生物标记基因调查领域仍面临着重大挑战：如何生成对社区最有用的数据，特别是在面对不断发展的测序技术和生物信息学管道时，以及如何纳入越来越多的目标基因。
