Katoh-Semba R, Semba R, Kashiwamata S, Kato K
Department of Perinatology, Institute for Developmental Research, Aichi Prefecture Colony, Japan.
Brain Res. 1990 Jul 2;522(1):112-7. doi: 10.1016/0006-8993(90)91584-4.
In our previous report, we have shown the sex difference in the concentration of the beta-subunit of nerve growth factor (beta-NGF) in the neural and paraneural tissues of mice. In this investigation, we examined the effects of castration of adult males, and of neonatal and/or adult treatments with testosterone on levels of beta-NGF in the several tissues of mice. Castration caused a marked reduction in the levels of serum testosterone and of beta-NGF in the brain, spinal cord and submandibular glands, but not in the pancreas and kidneys. Continuous infusion of testosterone for one week into adult males that had been castrated at 2 months of age restored the level of beta-NGF in the three tissues mentioned above. A single injection of testosterone to 5-day-old female pups to masculinize the brain gave no effect on the level of beta-NGF in any tissue dissected after 4 months. A one-week infusion of testosterone into adult females slightly increased levels of beta-NGF in the brain and spinal cord, but the same treatment of adult females given in advance a single dose of testosterone at 5 days of age caused a significant increase in its levels over those of untreated females. These results suggest that neonatal and adult exposures to testosterone can influence the endogenous concentration of beta-NGF in the brain and spinal cord.
在我们之前的报告中，我们已经表明小鼠神经组织和神经旁组织中神经生长因子β亚基（β-NGF）浓度存在性别差异。在本研究中，我们检测了成年雄性小鼠去势以及新生和/或成年期用睾酮处理对小鼠多个组织中β-NGF水平的影响。去势导致血清睾酮水平以及脑、脊髓和下颌下腺中β-NGF水平显著降低，但胰腺和肾脏中未出现这种情况。对2月龄时去势的成年雄性小鼠连续输注睾酮一周，可使上述三种组织中的β-NGF水平恢复。对5日龄雌性幼崽单次注射睾酮以使其脑雄性化，对4个月后解剖的任何组织中的β-NGF水平均无影响。对成年雌性小鼠输注睾酮一周可使脑和脊髓中β-NGF水平略有升高，但对5日龄时预先给予单剂量睾酮的成年雌性小鼠进行相同处理，其β-NGF水平比未处理的雌性小鼠显著升高。这些结果表明，新生期和成年期接触睾酮可影响脑和脊髓中β-NGF的内源性浓度。
