Somenarain L, Jakinovich W
Department of Biological Sciences, Lehman College, Bronx 10468.
Brain Res. 1990 Jul 2;522(1):83-9. doi: 10.1016/0006-8993(90)91580-a.
Antagonism of the gerbil's whole chorda tympani nerve taste responses by CuCl2 was studied. A 30 min pretreatment of 0.1 mM CuCl2 suppressed responses to single concentrations of the following sweeteners: L-alanine, L-proline, D-tryptophan, 6-chloro-D-tryptophan, L-valine, glycine, sucrose, maltose, lactose, tetrachloro-galacto-sucrose, glucose, fructose, methyl alpha-D-glucopyranoside, glycerol, sorbitol, sodium saccharin, L-4'-cyano-3'-(2-2-2-trifluoro acetamido)succinanilic acid, phenethyurea, and stevioside. The responses to L-serine and the starch hydrolysate, Polycose were depressed to a lesser degree. The responses to glycine HCl and NaCl were slightly suppressed by CuCl2. The 0.1 mM CuCl2 had no effect on the shape of the sucrose concentration-response curve or its 1/2 maximal response (CR50), but did suppress the maximum response (Rmax), characteristic of non-competitive antagonism. Our work suggests the presence of 2 separate receptor sites on the gerbil's taste receptor cell membrane, one of which interacts with sugar sweeteners and most other non-sugar sweeteners and the other with Polycose.
研究了氯化铜对沙鼠鼓索神经全神经味觉反应的拮抗作用。用0.1 mM氯化铜预处理30分钟可抑制对以下单一浓度甜味剂的反应：L-丙氨酸、L-脯氨酸、D-色氨酸、6-氯-D-色氨酸、L-缬氨酸、甘氨酸、蔗糖、麦芽糖、乳糖、四氯半乳蔗糖、葡萄糖、果糖、α-D-吡喃葡萄糖苷甲酯、甘油、山梨醇、糖精钠、L-4'-氰基-3'-(2-2-2-三氟乙酰胺)琥珀酰苯胺、苯乙脲和甜菊糖苷。对L-丝氨酸和淀粉水解产物聚葡萄糖的反应受到的抑制程度较小。氯化铜对甘氨酸盐酸盐和氯化钠的反应有轻微抑制作用。0.1 mM氯化铜对蔗糖浓度-反应曲线的形状或其半数最大反应(CR50)没有影响，但确实抑制了最大反应(Rmax)，这是非竞争性拮抗作用的特征。我们的研究表明，沙鼠味觉受体细胞膜上存在2个独立的受体位点，其中一个与糖类甜味剂和大多数其他非糖类甜味剂相互作用，另一个与聚葡萄糖相互作用。
