Poole Rachel L, Aleman Tiffany R, Ellis Hillary T, Tordoff Michael G
Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA
Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
Chem Senses. 2016 Jan;41(1):45-52. doi: 10.1093/chemse/bjv056. Epub 2015 Oct 12.
Rodents are strongly attracted to the taste(s) of maltodextrins. A first step toward discovery of the underlying genes involves identifying phenotypic differences among inbred strains of mice. To do this, we used 5-s brief-access tests and 48-h 2-bottle choice tests to survey the avidity for the maltodextrin, Maltrin M040, of mice from 8 inbred strains (129S1/SvImJ, A/J, CAST/EiJ, C57BL/6J, NOD/ShiLTJ, NZO/HlLtJ, PWK/PhJ, and WSB/EiJ). In brief-access tests, the CAST and PWK strains licked significantly less maltodextrin than equivalent concentrations of sucrose, whereas the other strains generally licked the 2 carbohydrates equally. Similarly, in 2-bottle choice tests, the CAST and PWK strains drank less 4% maltodextrin than 4% sucrose, whereas the other strains had similar intakes of these 2 solutions; the CAST and PWK strains did not differ from the C57, NOD, or NZO strains in 4% sucrose intake. In sum, we have identified strain variation in maltodextrin perception that is distinct from variation in sucrose perception. The phenotypic variation characterized here will aid in identifying genes responsible for maltodextrin acceptance. Our results identify C57 × PWK mice or NZO × CAST mice as informative crosses to produce segregating hybrids that will expose quantitative trait loci underlying maltodextrin acceptance and preference.
啮齿动物对麦芽糊精的味道有强烈的偏好。发现潜在基因的第一步是识别近交系小鼠之间的表型差异。为此，我们使用了5秒短暂接触试验和48小时双瓶选择试验，来调查8个近交系（129S1/SvImJ、A/J、CAST/EiJ、C57BL/6J、NOD/ShiLTJ、NZO/HlLtJ、PWK/PhJ和WSB/EiJ）小鼠对麦芽糊精Maltrin M040的喜好程度。在短暂接触试验中，CAST和PWK品系舔食麦芽糊精的量明显少于同等浓度的蔗糖，而其他品系通常对这两种碳水化合物的舔食量相同。同样，在双瓶选择试验中，CAST和PWK品系饮用4%麦芽糊精的量少于4%蔗糖，而其他品系对这两种溶液的摄入量相似；CAST和PWK品系在4%蔗糖摄入量上与C57、NOD或NZO品系没有差异。总之，我们已经确定了麦芽糊精感知方面的品系差异，这与蔗糖感知方面的差异不同。这里所描述的表型变异将有助于识别负责麦芽糊精接受的基因。我们的结果确定C57×PWK小鼠或NZO×CAST小鼠为有信息价值的杂交组合，可产生分离杂种，从而揭示麦芽糊精接受和偏好背后的数量性状位点。
