Laboratory of Scientific Research and Experimental Surgery, Second Propedeutical Department of Surgery, Medical School, Aristotle University, and Hippokration General Hospital, Thessaloniki, Greece.
Br J Surg. 2012 Mar;99(3):423-9. doi: 10.1002/bjs.7817. Epub 2012 Jan 13.
Intraperitoneal adhesions are a common problem in abdominal surgery. The aim of this study was to compare the effectiveness of Statofilm, a novel antiadhesive film based on cross-linked carboxymethylcellulose and atorvastatin, with that of sodium hyaluronate-carboxymethylcellulose (Seprafilm(®)) in the prevention of postoperative intraperitoneal adhesions in rats.
One hundred male Wistar rats underwent a laparotomy and adhesions were induced by caecal abrasion. The animals were allocated to five groups: a control group with no adhesion barrier, Seprafilm(®) group, placebo group with a film containing carboxymethylcellulose without atorvastatin, and low- and high-dose groups with films containing carboxymethylcellulose and atorvastatin 0·125 and 1 mg per kg bodyweight respectively. Adhesions were classified by two independent surgeons 2 weeks after surgery. Caecal biopsies were obtained for histological evaluation of fibrosis, inflammation and vascular proliferation.
All antiadhesive film groups (Seprafilm(®), placebo, low-dose and high-dose) had statistically significant adhesion reduction compared with the control group (P < 0·001, P = 0·015, P < 0·001 and P < 0·001 respectively). The low-dose Statofilm was superior to Seprafilm(®) in terms of adhesion prevention (P = 0·001). Adhesions were present in three-quarters of rats in the Seprafilm(®) group, but only one-quarter in the low-dose Statofilm group.
The data suggest that the newly developed adhesion barrier Statofilm has better results than Seprafilm(®) in preventing postoperative adhesions in rats. A low-dose atorvastatin-containing film, such as Statofilm, could be evaluated for future clinical application.
腹腔粘连是腹部手术中的一个常见问题。本研究旨在比较新型基于交联羧甲基纤维素和阿托伐他汀的防粘连膜 Statofilm 与透明质酸钠羧甲基纤维素（Seprafilm）预防大鼠术后腹腔粘连的效果。
100 只雄性 Wistar 大鼠行剖腹术，并通过盲肠摩擦诱导粘连。将动物分为五组：无粘连屏障的对照组、Seprafilm 组、含无阿托伐他汀羧甲基纤维素的安慰剂组、含羧甲基纤维素和阿托伐他汀 0.125 和 1 mg/kg 体重的低剂量和高剂量组。术后 2 周，由两位独立外科医生对粘连进行分类。取盲肠活检，进行纤维化、炎症和血管增殖的组织学评估。
所有防粘连膜组（Seprafilm、安慰剂、低剂量和高剂量）与对照组相比，粘连程度均有统计学显著降低（P<0.001，P=0.015，P<0.001 和 P<0.001）。低剂量 Statofilm 在预防粘连方面优于 Seprafilm（P=0.001）。Seprafilm 组中四分之三的大鼠存在粘连，而低剂量 Statofilm 组中仅有四分之一的大鼠存在粘连。
数据表明，新开发的粘连屏障 Statofilm 在预防大鼠术后粘连方面的效果优于 Seprafilm。可以评估含低剂量阿托伐他汀的 Statofilm 膜用于未来的临床应用。
