Dyke G W, Craven J L, Hall R, Cooper D P, Soballa G, Garner R C
Cancer Research Unit, University of York, Heslington, U.K.
Cancer Lett. 1990 Nov 5;54(3):147-51. doi: 10.1016/0304-3835(90)90036-w.
The spectrum of activity of the DNA repair enzyme O6-alkyltransferase has been studied in a large series of normal stomachs in order to establish the baseline range of values for this enzyme. Sixty-eight patients with histologically normal stomachs were biopsied during the course of upper gastrointestinal endoscopy and the biopsies assayed for O6-alkyl-transferase activity. A wide spectrum of activity was found with values ranging from 38 fmol O6-guanine extracted/mg protein to over 400 fmol/mg. This suggests that there may be wide inter-individual differences in susceptibility to alkylating actions in the human gastric mucosa.
为了确定DNA修复酶O6-烷基转移酶活性的基线值范围，对大量正常胃组织进行了活性谱研究。68例组织学检查显示胃正常的患者在接受上消化道内镜检查时进行了活检，并对活检组织检测了O6-烷基转移酶活性。结果发现活性范围很广，从每毫克蛋白质提取38飞摩尔O6-鸟嘌呤到超过400飞摩尔/毫克。这表明人类胃黏膜对烷化作用的易感性可能存在很大的个体差异。
