Ueo H, Matsuoka H, Sugimachi K, Kuwano H, Mori M, Akiyoshi T
Department of Surgery, Kyushu University, Beppu, Japan.
Cancer Res. 1990 Nov 15;50(22):7212-5.
In order to accurately determine sex hormone dependency and hormone responsiveness in human esophageal carcinoma, the effects of sex hormones on the growth of esophageal carcinoma cell lines, KSE-1 and KSE-2 cells, were examined in vitro and in vivo. Cell proliferation of cultured KSE-1 cells was inhibited by treatment of estradiol and enhanced by dihydrotestosterone (DHT), whereas KSE-2 cells were unaffected by these sex hormones. The heterotransplanted tumors of KSE-1 cells in nude mice possessed estrogen receptor (ER) and androgen receptor (AR), while the tumors of the KSE-2 cells had neither ER nor AR. When the tumor growth rates and serum hormone levels were monitored during the continuous administration of either estradiol or DHT, no significant differences were observed in either the serum hormone levels or tumor growth rates between male and female mice. The administration of estradiol significantly inhibited the growth of ER-positive and AR-positive KSE-1 tumors in both males and females in conjunction with an increase in the estradiol levels and a decrease in the DHT levels in the serum. However, the growth of ER-negative and AR-negative KSE-2 tumors was not influenced by either estradiol or DHT administration. These results suggest that the in vivo growth of human esophageal carcinoma cells with sex hormone receptor is influenced by circulating hormone levels and can be manipulated by systemic estradiol administration.
为了准确确定人类食管癌中的性激素依赖性和激素反应性，在体外和体内研究了性激素对食管癌细胞系KSE-1和KSE-2细胞生长的影响。培养的KSE-1细胞的细胞增殖受到雌二醇处理的抑制，并被双氢睾酮（DHT）增强，而KSE-2细胞不受这些性激素的影响。KSE-1细胞在裸鼠中的异种移植肿瘤具有雌激素受体（ER）和雄激素受体（AR），而KSE-2细胞的肿瘤既没有ER也没有AR。当在连续给予雌二醇或DHT期间监测肿瘤生长速率和血清激素水平时，雄性和雌性小鼠之间的血清激素水平或肿瘤生长速率均未观察到显著差异。雌二醇的给药显著抑制了雄性和雌性中ER阳性和AR阳性的KSE-1肿瘤的生长，同时血清中雌二醇水平升高，DHT水平降低。然而，ER阴性和AR阴性的KSE-2肿瘤的生长不受雌二醇或DHT给药的影响。这些结果表明，具有性激素受体的人类食管癌细胞的体内生长受循环激素水平的影响，并且可以通过全身给予雌二醇来控制。
