Matsuoka H, Sugimachi K, Ueo H, Kuwano H, Nakano S, Nakayama M
Cancer Res. 1987 Aug 1;47(15):4134-40.
Biopsy tissues from a 68-year-old Japanese man with metastases to axillary lymph nodes of a recurrent esophageal carcinoma were adapted to cell culture conditions and a continuously growing tumor cell line was developed. Immunohistochemical staining revealed that these cells contained keratinous material and the electron microscopic study revealed the presence of tonofilaments. Thus, this line, designated the KSE-1 line, was considered to have originated from metastatic squamous cell carcinoma of the esophagus. This line has a binding content of 4.2 fmol/mg protein for the estrogen receptor and 2.2 fmol/mg protein for the testosterone receptor. By measurement of cell number and thymidine incorporation, the growth rate of this line was found to be moderately responsive to these hormones, being inhibited by estrogen and enhanced by testosterone at concentration levels between 10(-13) and 10(-8) and 10(-13) and 10(-6) mg/ml, respectively.
一名68岁日本男性复发性食管癌伴腋窝淋巴结转移，从其活检组织中培养出适应细胞培养条件的细胞，并建立了持续生长的肿瘤细胞系。免疫组织化学染色显示这些细胞含有角质物质，电子显微镜研究显示存在张力丝。因此，该细胞系命名为KSE-1系，被认为起源于食管转移性鳞状细胞癌。该细胞系雌激素受体结合含量为4.2 fmol/mg蛋白，睾酮受体结合含量为2.2 fmol/mg蛋白。通过细胞计数和胸腺嘧啶核苷掺入量测量，发现该细胞系的生长速率对这些激素有中度反应，分别在10(-13)至10(-8)mg/ml和10(-13)至10(-6)mg/ml浓度水平下，雌激素抑制其生长，睾酮促进其生长。
