Kao P C, Jiang N S, Carpenter P C
Clin Chem. 1979 Jul;25(7):1267-73.
A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol in conjunction with a second precipitating antibody for fast (15-min) separation of antibody-bound and free corticotropin. The assay had a sensitivity of 16 ng/L and was validated on patients with various pituitary and adrenal diseases. From 103 normal subjects, the median value for corticotropin in specimens collected during the morning was 34 ng/L of plasma; the upper 95% confidence limit of the normal range was 98 ng/L.
用与牛血清白蛋白偶联的合成1-24促肾上腺皮质激素免疫家兔，获得了促肾上腺皮质激素抗血清。该抗血清与合成α-促黑素或合成β-内啡肽无交叉反应，与人β-促脂素的交叉反应率为0.23%。我们用所获得的抗血清开发了一种放射免疫测定法，其中我们使用聚乙二醇结合第二种沉淀抗体，以便快速（15分钟）分离结合抗体和游离促肾上腺皮质激素。该测定法的灵敏度为16 ng/L，并在患有各种垂体和肾上腺疾病的患者身上得到验证。在103名正常受试者中，上午采集的标本中促肾上腺皮质激素的中位数为血浆34 ng/L；正常范围的95%置信上限为98 ng/L。
