Department of Fixed and Implant Prosthodontics, Genoa University, Genova, Italy.
Clin Oral Implants Res. 2013 Aug;24(8):880-9. doi: 10.1111/j.1600-0501.2011.02397.x. Epub 2012 Jan 17.
The aim of this study was an in vitro comparison of osteoblast adhesion, proliferation and differentiation related to six dental implants with different surface characteristics, and to determine if the interaction between cells and implant is influenced by surface structure and chemical composition.
Six types of implants were tested, presenting four different surface treatments: turned, sandblasted, acid-etched, anodized. The implant macro- and microstructure were analyzed using SEM, and the surface chemical composition was investigated using energy-dispersive X-ray analysis. SaOS-2 osteoblasts were used for the evaluation of cell adhesion and proliferation by SEM, and cell viability in contact with the various surfaces was determined using cytotoxicity MTT assays. Alkaline phosphatase (ALP) enzymatic activity in contact with the six surfaces was evaluated. Data relative to MTT assay and ALP activity were statistically analyzed using Kruskal-Wallis not parametric test and Nemenyi-Damico-Wolfe-Dunn post hoc test.
All the implants tested supported cell adhesion, proliferation and differentiation, revealing neither organic contaminants nor cytotoxicity effects. The industrial treatments investigated changed the implant surface microscopic aspect and SaOS-2 cell morphology appeared to be influenced by the type of surface treatment at 6, 24, and 72 h of growth. SaOS-2 cells spread more rapidly on sandblasted surfaces. Turned surfaces showed the lowest cell proliferation at SEM observation. Sandblasted surfaces showed the greatest ALP activity values per cell, followed by turned surfaces (P < 0.05).
On the base of this in vitro investigation, differently surfaced implants affected osteoblast morphology, adhesion, proliferation, and differentiation. Sandblasted surfaces promoted the most suitable osteoblast behavior.
本研究旨在比较 6 种不同表面特性的牙科种植体的成骨细胞黏附、增殖和分化情况，以确定细胞与种植体的相互作用是否受表面结构和化学成分的影响。
对 6 种种植体进行测试，它们呈现出四种不同的表面处理：车削、喷砂、酸蚀、阳极氧化。使用扫描电子显微镜（SEM）分析种植体的宏观和微观结构，并使用能量色散 X 射线分析（EDX）研究表面化学成分。使用 SEM 评估 SaOS-2 成骨细胞的黏附力和增殖能力，并通过细胞毒性 MTT 测定确定与各种表面接触的细胞活力。评估与 6 种表面接触的碱性磷酸酶（ALP）酶活性。使用 Kruskal-Wallis 非参数检验和 Nemenyi-Damico-Wolfe-Dunn 事后检验对 MTT 检测和 ALP 活性数据进行统计学分析。
所有测试的种植体均支持细胞黏附、增殖和分化，既没有显示出有机污染物，也没有细胞毒性作用。研究的工业处理改变了种植体表面的微观外观，SaOS-2 细胞形态似乎受到表面处理类型的影响，在培养 6、24 和 72 小时时。喷砂表面使 SaOS-2 细胞更快地扩散。车削表面在 SEM 观察时显示出最低的细胞增殖。喷砂表面的每个细胞的 ALP 活性值最大，其次是车削表面（P<0.05）。
基于这项体外研究，不同表面处理的种植体影响成骨细胞形态、黏附、增殖和分化。喷砂表面促进了最适合的成骨细胞行为。
